Study on effects and mechanism of Qifu Lizhong Enema Prescription on mechanical barrier function of intestinal mucosa in rats with ulcerative colitis
10.3760/cma.j.cn115398-20230807-00062
- VernacularTitle:芪附理中灌肠方对溃疡性结肠炎模型大鼠肠黏膜机械屏障的影响及机制研究
- Author:
Wei LI
1
;
Lingling YUAN
;
Jiaxin LI
;
Pengfei WEI
;
Shuangyuan HU
;
Yanwei HAO
;
Yi ZHANG
Author Information
1. 成都中医药大学附属医院消化内科，成都 610075
- Keywords:
Colitis, ulcerative;
Qifu Lizhong Enema Prescription;
Tight junctions;
Junction adhesion molecule A;
Rats
- From:
International Journal of Traditional Chinese Medicine
2024;46(7):874-880
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Qifu Lizhong Enema Prescription on ulcerative colitis rats with yang deficiency of spleen and kidney syndrome; To discuss its mechanism.Methods:Totally 70 male SD rats were randomly divided into blank group, model group, mesalazine group, Qifu Lizhong Guanchang Prescription high-, medium- and low-dosage groups; blank group ( n=10), other groups ( n=12). Except for the blank group, the other groups used bitter cold purgative therapy (Dahuang Decoction) by gavage, and combined with trinitrobenzen sulfonic acid (TNBS) +55% ethanol compound method to induce UC rat model. After successful modeling, the blank group and model group were given 1 ml normal saline enema daily, Qifu Lizhong Enema Prescription groups were given Qifu Lizhong Enema Prescription 3.00, 1.50, 0.75 g/kg enema daily, and the mesalazine group was given mesalazine 0.03 g/kg enema daily, once a day for consecutive 14 days. After 14 days, Disease Activity Index (DAI) score was performed, and hematoxylin-eosin staining (HE) was used to observe the pathological tissues of the colon. The expressions of Occludin and adhesion molecules A (JAM-A) protein in colon tissue were detected by immunohistochemistry and Western blot. Results:HE results showed that the mucosal structure was damaged, inflammatory cells were infiltrated, edema and ulcer foci were observed in model group. The mucosal structure of mesalazine group and Qifu Lizhong Enema Prescription groups were intact, and inflammatory infiltration, edema and ulcer of neoepithelial were improved. Compared with model group, the DAI scores of Qifu Lizhong Enema Prescription groups decreased ( P<0.01), the expressions of Occludin and JAM-A in Qifu Lizhong Guanchang Prescription high- and medium-dosage groups significantly increased ( P<0.05). Conclusion:Qifu Lizhong Enema Prescription can significantly relieve the symptoms and pathological morphology of UC rats, and the mechanism of repairing intestinal mucosal barrier may be related to up-regulating the expressions of Occludin and JAM-A proteins.
