Correlation analysis on HPLC characteristic spectrum of Centipeda minima based on standard decoction
10.3760/cma.j.cn115398-20230905-00037
- VernacularTitle:基于标准汤剂的鹅不食草HPLC特征图谱相关性研究
- Author:
Huaming LIU
1
;
Xiaoying ZHENG
;
Hui ZHANG
;
Pei TAN
;
Yuhua LIU
Author Information
1. 华润三九医药股份有限公司，深圳 518110
- Keywords:
Centipeda minima;
Standard Decoction;
Characteristic spectrum;
Correlation;
Formula Granules
- From:
International Journal of Traditional Chinese Medicine
2024;46(7):905-912
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a HPLC characteristic spectrum method of Centipeda minima based on standard decoction; To evaluate the correlation of materia medica, decoction pieces, standard decoction, intermediates and formula granule; To provide a reference for the quality standard research of Centipeda minima formula granules.Methods:The materia medica, decoction pieces, standard decoction, intermediates and formula granules of Centipeda minima were analyzed by a HPLC characteristic spectrum method, and the software "Similarity Evaluation System for Chromatographic Fingerprint of TCM" was employed to conform the common peaks. The clustering analysis (CA), principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were adopted to find out the correlation of materia medica, decoction pieces, standard decoction, intermediates and formula granules of Centipeda minima. The Iconic components which can make differences was screened at the same time.Results:The characteristic chromatogram of materia medica, decoction pieces, standard decoction, intermediates and formula granules of Centipeda minima shared high similarity with the respective control chromatogram. Fourteen common peaks were confirmed, and eleven chromatographic peaks were identified. The results of CA and PCA were consistent, and the standard decoction, intermediates and formula granules of Centipeda minima were closer. Six differential components with VIP values greater than 1 were screened by OPLS-DA.Conclusions:The method is stable, accurate and characteristic. The study can provide a reference for the quality standard research of Centipeda minima Formula Granules by analyzing the correlation of characteristic spectrum of materia medica, decoction pieces, standard decoction, intermediates and formula granules.
