Study on quantitative transfer relationship of Radix Actinidiae Chinensis formula granules based on standard decoction
10.3760/cma.j.cn115398-20240201-00003
- VernacularTitle:以标准汤剂为基准的猕猴桃根配方颗粒量值传递研究
- Author:
Dan ZHANG
1
;
Jian JIN
;
Lin CHEN
;
Bingbing SHEN
;
Liangzi FANG
;
Hui TU
;
Shuihan ZHANG
Author Information
1. 湖南中医药大学2021级硕士研究生，长沙 410208
- Keywords:
Radix Actinidiae Chinensis;
Catechin;
Standard decoction;
Formula granules;
Characteristic chromatogram;
Transfer rate;
Quantitative transfer relationship
- From:
International Journal of Traditional Chinese Medicine
2024;46(6):757-763
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a high-performance liquid chromatography (HPLC) characteristic spectrum of Radix Actinidiae Chinensis decoction pieces, standard decoction and formula granules; To simultaneously determine the contents of index components; To study the transfer law of formula granules based on the extraction amount, content of Catechin and characteristic spectrum of standard decoction.Methods:15 batches of Radix Actinidiae Chinensis decoction pieces were collected, the standard decoction was prepared, and the extraction amount was measured. The HPLC fingerprints of decoction pieces, standard decoction and formula granules were established, and the characteristic peaks were calibrated and attributed. The content of Catechins in decoction pieces, standard decoction and formula granules was measured, and the transfer rate law was calculated. The yield rate, the common peak transmission number of fingerprints, the content and the transfer rate were the main evaluation indexes, and the law of the transmission of the magnitude value was analyzed.Results:The paste yield of 15 batches of Radix Actinidiae Chinensis standard decoction ranged from 3.9%-6.3%. A total of 4 characteristic peaks were calibrated in the feature map, and peak 2 was identified as Protocatechuic acid and peak 4 as Catechin; 6 characteristic peaks were detected in Actinidia chinensis Planchon decoction pieces, standard decoction and formula granules, and their relative retention times were all within the specified range. The content of Catechins in Radix Actinidiae Chinensis decoction pieces was 0.4%-1.4%, which was 3.8%-4.9% in formula granules. The transfer rate of decoction pieces-standard decoction was 13.6%-38.3%, and the decoction-formula granules was 15.5%-21.2%.Conclusions:The mass transfer between Radix Actinidiae Chinensis decoction pieces, standard decoction and formula granules has a good migration. The formula granules also have a good correlation and consistency with the standard decoction, which indicating that the preparation process of Radix Actinidiae Chinensis is reasonable and feasible.
