Exploration on the mechanism of acupuncture and moxibustion combined with sand therapy on bone biomechanics and immune function of knee osteoarthritis rabbits based on G protein coupled signaling pathway
10.3760/cma.j.cn115398-20230807-00064
- VernacularTitle:基于G蛋白偶联信号通路探讨针灸联合沙疗对膝骨关节炎家兔骨生物力学及免疫功能的作用机制
- Author:
Mingjun XU
1
;
Liangyu QIU
;
Chan WANG
;
Cheng CHEN
;
Xueping ZHU
Author Information
1. 十堰市太和医院/湖北医药学院附属医院针灸科，十堰 442000
- Keywords:
Osteoarthritis, knee;
Acupuncture moxibustion therapy;
Ammotherapy;
Bone biomechanics;
Immunoglobulins;
Rabbits
- From:
International Journal of Traditional Chinese Medicine
2024;46(6):737-742
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of acupuncture combined with sand therapy on bone biomechanics and immune function in rabbits with knee osteoarthritis based on G protein coupled signal.Methods:Totally 35 rabbits were randomly divided into normal group, model group, sand therapy group, acupuncture and moxibustion group and combined group, with 7 rabbits in each group. Except for the normal group, all other groups were prepared with knee osteoarthritis models. After 12 h modeling, rabbits in the sand therapy group and the combined group were treated with sand therapy. Rabbits in the acupuncture and moxibustion group and the combined group received acupuncture stimulation intervention therapy. Rabbits in normal group and model group only moved freely. The levels of IgG, IgM and IgA in serum and IL-1β and TNF-α were detected by ELISA method. The biomechanical properties of femur were measured by American Instron universal material experimental machine. The pathological changes of rabbits in each group were observed by HE staining. Protein expression levels of Gαs, cAMP and Gi in rabbit cartilage tissues were detected by Western blot.Results:Compared with model group, the levels of IgG, IgM and IgA increased ( P<0.05)l the levels of IL-1β and TNF-α decreased ( P<0.05); the maximum load, breaking load, structural stiffness and capacity absorption of rabbits in sand therapy group, acupuncture group and combined group increased ( P<0.05); the expressions of Gαs and cAMP protein increased ( P<0.05), while the expression of Gi protein decreased ( P<0.05). Conclusion:Acupuncture combined with sand therapy in the treatment of knee osteoarthritis can effectively improve the bone strength of osteoarthritis, reduce the inflammatory response, balance the expression of G protein and improve the imbalance of immunoglobulin.
