Study on the quality of Mori Ramulus from different producing areas based on HPLC fingerprinting combined with chemical pattern recognition
10.3760/cma.j.cn115398-20231124-00284
- VernacularTitle:HPLC指纹图谱结合化学模式识别研究不同产地桑枝质量
- Author:
Yanru ZHOU
1
;
Yaqi LIU
;
Zuohua XIE
;
Lixiang ZHOU
;
Wensheng SHENG
Author Information
1. 江西中医药大学药学院，南昌 330004
- Keywords:
Mulberry branch;
Content determination;
Fingerprints;
Chemical pattern recognition;
Quality evaluation
- From:
International Journal of Traditional Chinese Medicine
2024;46(6):750-756
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish HPLC fingerprint spectra and multi-component content determination methods for 20 batches of Mori Ramulus from different origins; To evaluate the quality of Mori Ramulus from different producing areas with similarity evaluation, CA, PCA, PLS-DA and other chemical pattern recognition methods.Methods:The chromatography was performed on Wondasil C18 column (4.6 mm×250 mm, 5 μm) at the detection wavelength of 254 nm, with acetonitrile (A) -0.2% glacial acetic acid (B) as the mobile phase at the flow rate of 0.8 ml/min. The fingerprint was established through the "TCM chromatographic Fingerprint Similarity Evaluation System", the similarity was calculated, and the chemical pattern recognition was carried out by SPSS 26.0 and SIMCA 14.1 software.Results:The similarity calculation results of fingerprint spectra showed that the similarity of the production areas in Fujian, Zhejiang, and Hebei was>0.830, while the similarity of other production areas was>0.900; a total of 14 common peaks were identified, and 8 components of mulberroside A, chlorogenic acid, polydatin, oxyresveratrol, resveratrol, morin, kuwanone G and morusin were identified; CA, PCA analysis divided Mori Ramulus from different producing areas into two categories, extracted four principal components, and the cumulative variance contribution rate was 89.247%. PLS-DA analysis was based on a VIP value greater than 1, and three quality difference criteria of mulberroside A, kuwanone G and morusin were selected.Conclusions:The established HPLC fingerprint and content determination method is simple, reproducible, stable, accurate and reliable. Combined with the chemical stoichiometric analysis methods, the quality evaluation of Mori Ramulus from different origins can be complete, and provide a reference for the quality control of mulberry branches.
