Establishment of UPLC characteristic chromatogram of Pulsatilla chinensis and its application in origin differentiation and counterfeit identification
10.3760/cma.j.cn115398-20230629-00328
- VernacularTitle:白头翁药材UPLC特征图谱的建立及其在产地区分与伪品鉴别中的应用
- Author:
Guangming HE
1
;
Rui LUO
;
Heping ZENG
;
Xiaoying LU
;
Xiaolong YANG
;
Weisheng LYU
;
Yueyi LIANG
;
Zhenyu LI
;
Dongmei SUN
;
Xiangdong CHEN
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Pulsatillae Radi x;
UPLC characteristic chromatogram;
Principal component analysis;
Orthogonal partial least squares-discriminant analysis;
Place of
- From:
International Journal of Traditional Chinese Medicine
2024;46(6):743-749
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish ultra performance liquid chromatography (UPLC) characteristic chromatogram of Pulsatilla chinensis; To provide reference for the origin identification and quality control of Pulsatilla chinensis. Methods:UPLC Method was adopted. The determination was performed on a column of Agilent SB C18 (2.1 mm×100 mm, 1.8 μm) . The mobile phase was acetonitrile-methanol (2:1) -0.1% phosphoric acid solution by fradient elution at a flow rate of 0.30ml/min. The column temperature was 30 ℃. The detection wavelength was 215 nm. The injection volume was 2 μl. The common counterfeit products and medicinal herbs of Pulsatilla chinensis from different areas were evaluated by comparison of characteristic chromatogram, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Results:There were 9 characteristic peaks in the characteristic chromatogram of Pulsatilla chinensis, and 8 common peaks were identified by high resolution mass spectrometry and comparison of reference materials. Through PCA analysis, it was possible to clearly distinguish the medicinal herbs of Pulsatilla chinensis from different areas. Combined with OPLS-DA analysis, it was found that peak 2, peak 3, peak 6 were the main markers of Pulsatilla chinensis from different producing areas. Conclusion:The established method has good specificity, repeatability and durability, and it can effectively distinguish the common counterfeits of Pulsatilla chinensis, and provide the basis of quality control and selection of origin for Pulsatilla chinensis.
