Two-sample Mendelian randomization study of gut microbiota and lung function (FEV1/FVC) and the thought on its application in the TCM field
10.3760/cma.j.cn115398-20230612-00146
- VernacularTitle:肠道微生物和肺功能（FEV1/FVC）的两样本孟德尔随机化研究及其在中医药领域应用的思考
- Author:
Xurui HUANG
1
;
Zhen MA
;
Xiaoning LI
;
Qifan ZHANG
;
Xinyan WAN
;
Haomin ZHENG
;
Yu ZHANG
;
Honghong WANG
Author Information
1. 北京中医药大学第三临床医学院健康管理中心，北京 100029
- Keywords:
Mendelian randomization analysis;
Gastrointestinal microbiome;
Pulmonary function;
Causality;
Traditional Chinese Medicine
- From:
International Journal of Traditional Chinese Medicine
2024;46(6):698-706
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the causal relationship between gut microbiota and lung function (FEV1/FVC) using two-sample Mendelian randomization method; To explore its application in the TCM field.Methods:This was a Mendelian randomization study. The GWAS data of gut microbiota from the MiBioGen consortium study and the GWAS data of lung function (FEV1/FVC) published by IEU OpenGWAS in the public database were used, and instrumental variables were extracted according to prespecified thresholds. The inverse variance weighted method (IVW) was mainly used for analysis. The results were evaluated according to the effect indicator β value and 95% CI. When the IVW method was statistically significant, further sensitivity analysis was performed. Leave-one-out test, heterogeneity test, horizontal gene pleiotropy test and MR-Egger regression intercept analysis were used to verify the stability and reliability of the results. Results:A total of 10 causal relationships between gut microbiota and lung function (FEV1/FVC) were determined using the IVW method: family. BacteroidalesS24.7group ( β=-0.029, P=0.015), family. ClostridialesvadinBB60group ( β=-0.028, P=0.040), family. Streptococcaceae ( β=-0.056, P=0.042), genus. LachnospiraceaeFCS020group ( β=0.025, P=0.029), genus. Lactococcus ( β=-0.024, P=0.038), genus. Peptococcus ( β=0.025, P=0.049), genus. RuminococcaceaeUCG011 ( β=-0.030, P=0.038), genus. Ruminococcus2 ( β=0.028, P=0.033), genus. Terrisporobacter ( β=-0.030, P=0.018), phylum. Cyanobacteria ( β=0.027, P=0.039). Leave-one-out analysis showed that the results were stable, and the effects of heterogeneity and horizontal gene pleiotropy on causal effect estimation could be removed. Conclusion:The gut microbiota may play a role in the changes of lung function, which to a certain extent confirms the TCM theory of "exterior-interior relationship between the lung and large intestine", and can provide certain reference for the research direction of TCM.
