Simultaneous determination of 6 components in Tongluo Zhibi Prescription by HPLC wavelength switching method
10.3760/cma.j.cn115398-20231106-00052
- VernacularTitle:HPLC波长切换法同时测定通络治痹方中6种成分含量
- Author:
Xue ZHANG
1
;
Xia LEI
;
Deping ZHAO
;
Ziyue ZHU
;
Zhuoyi HU
;
Guoda DAI
;
Wenjie GE
;
Zhenhua BIAN
;
Yafeng ZHANG
;
Ning ZHANG
Author Information
1. 佳木斯大学药学院，佳木斯 154007
- Keywords:
Assay (TCD);
Tongluo Zhibi Recipe;
High performance liquid chromatography;
Conversion wavelength method
- From:
International Journal of Traditional Chinese Medicine
2024;46(5):637-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish high performance liquid chromatography (HPLC) wavelength switching method to simultaneously determine the contents of chlorogenic acid, hydroxysafflor yellow A, ferulic acid, Nicotiflorin, Osthole and columbianadin in Tongluo Zhibi Prescription.Methods:The column was XBridge C18 column (250 mm × 4.6 mm, 5 μm); the mobile phase was acetonitrile (A)-0.1% phosphate water (B); gradient eluted, with flow rate: 1 ml/min, column temperature: 30 °C, detection wavelength 330 nm (0-14 min detection of chlorogenic acid, 15-80 min detection of ferulic acid, Nicotiflorin, Osthole, and columbianadin), 403 nm (14-15 min detection of hydroxysafflower yellow pigment A).Results:Chlorogenic acid, hydroxyrhodopsin A, ferulic acid, kaempferol 3-O-rutinoside, serpentin, and dihydroeurobicarpus angelicus acid ester showed good linearity ( R2 ≥ 0.999 8) within 0.029 7-1.485 0, 0.030 0-1.500 0, 0.009 9-0.495 0, 0.017 5-0.875 0, 0.028 4-1.420 0, 0.013 7-0.685 0 μg, respectively. The precision, stability (24 h), repeatability relative standard deviation ( RSD) were all <2%. The average spiked recoveries were all in the range of 95%-105%, and the RSDs were all in the range of 0.32%-1.67%. In 10 batches of test samples of Tongluo Zhibi Prescription, the content of the above six components, including chlorogenic acid, was determined to be 0.221 60, 0.314 30, 0.085 10, 0.032 95, 0.043 87, 0.026 21 mg/g in the following order. Conclusion:The established HPLC wavelength switching method is fast, simple and accurate, which can be used for simultaneous determination of the content of the above six components in Tongluo Zhibi Prescription, which provides reference for quality monitoring and new dosage form research of Tongluo Zhibi Prescription.
