Exploration on mechanism of Shenghui Yizhi Decoction in improving learning and memory ability of SAMP8 mice based on NLRP3/ Caspase-1/ IL-1β signaling pathway
10.3760/cma.j.cn115398-20230706-00067
- VernacularTitle:基于NLRP3/ Caspase-1/ IL-1β通路探讨生慧益智汤改善SAMP8小鼠学习记忆能力的作用机制
- Author:
Peng WANG
1
;
Yuanli DONG
;
Weiliang SUN
;
Yi JIN
;
Rong MEI
;
Baosheng ZHAO
;
Qiubing LI
Author Information
1. 中日友好医院保健部二部，北京 100029
- Keywords:
Alzheimer disease;
Shenghui Yizhi Decoction;
NLRP3 inflammasome;
Neuroinflammation;
Coordinating heart and kidney;
Mice
- From:
International Journal of Traditional Chinese Medicine
2024;46(5):594-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Shenghui Yizhi Decoction on learning and memory ability of SAMP8 mice; To explore its mechanism.Methods:Totally 24 male SAMP8 mice were randomly divided into model group, donepezil hydrochloride group (1 mg/kg) and Shenghui Yizhi Decoction group (15.6 g/kg), with 8 mice in each group, and eight anti rapid aging mice (SAMR1) of the same age and strain were set as the control group. The mice in each group were given corresponding medication or the same volume of distilled water for 90 days. After administration, Morris water maze was used to detect the learning and memory ability and space exploration ability of mice. Immunohistochemistry was used to detect expression of β Amyloid protein 1-42 (Aβ 1-42) in hippocampal. ELISA was used to detect the levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Real time PCR was used to detect expressions of NOD-like receptor thermal protein domain associated protein 3 (NLRP3), cysteine protease-1 (Caspase-1), and IL-1β mRNA. Western Blot was used to detect expression of NLRP3, Caspase-1and IL-1β protein. Results:Compared with model group, the spatial exploration ability and memory ability of mice in Shenghui Yizhi Decoction group and donepezil hydrochloride group were improved ( P<0.05, P<0.01), the expression of Aβ 1-42 in hippocampus decreased ( P<0.05, P<0.01), the contents of IL-1β, IL-6 and TNF-α decreased ( P<0.05, P<0.01), and the mRNA and protein expressions of NLRP3, Caspase-1 and IL-1β decreased ( P<0.05, P<0.01). Conclusion:Shenghui Yizhi Decoction can effectively improve the learning and memory ability of SAMP8 mice, and its mechanism may be related to inhibiting the activation of inflammasome and neuroinflammatory response by regulating the NLRP3/ Caspase-1/ IL-1β signaling pathway.
