Analysis of p53 Gene Mutation in Basal Cell Carcinoma in Korean Patients.
- Author:
Chang Keun OH
1
;
Jun Ho LEE
;
Jae Young LIM
;
Sung Jun KIM
;
Moon Bum KIM
;
Ho Sun JANG
;
Kyung Sool KWON
Author Information
1. Department of Dermatology, College of Medicine, Pusan National University, Pusan, Korea. kwonks@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Basal cell carcinoma;
p53 mutation;
Korean
- MeSH:
Carcinoma, Basal Cell*;
Exons;
Genes, p53*;
Humans;
Introns;
Paraffin;
Ribonucleases
- From:Korean Journal of Dermatology
2002;40(6):651-659
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: There were few data on the gene mutations involved in the development of basal cell carcinoma(BCC) in Korean. OBJECTIVE: To gain insight into the role of p53 gene mutations of BCC in Koreans. METHODS: Fifteen BCCs were screened for mutation of the p53 gene. Immunohistochemical staining was done on the paraffin sections using a labelled streptavidin-biotin-peroxidase complex method with the primary antibody against p53 protein. Analysis of p53 mutation was done by non-isotopic RNase cleavage assay and direct sequencing. RESULTS: Mutation of p53 gene was found in 40%(6/15) of the cases. One case showed mutations in exon 6, one in exon 6 and intron 8, two in exon 8, one in exon 9, and one in intron 5. But ultraviolet specific C->T change was detected in only one case. Immunohistochemical expression of p53 was seen in 40%(6/15), but its expression did not coincide with p53 gene mutation. CONCLUSION: Ultraviolet specific mutations of p53 were much less frequent in Korean than in Caucasian, suggesting other etiologies than ultraviolet radiation.
