Evaluation of a Rapid Immunochromatographic Assay Kit for the Diagnosis of Salmonella Typhi Infection.
- Author:
Sun Min LEE
1
;
Young Jin KIM
;
Sang Hyun HWANG
;
Eun Yup LEE
;
Han Chul SON
;
Chulhun L CHANG
Author Information
1. Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea. CCHL@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Salmonella Typhi;
Immunochromatographic assay;
Typhoid fever;
Widal test
- MeSH:
Bacteremia;
Diagnosis*;
Gyeonggi-do;
Humans;
Immunochromatography*;
Incidence;
Korea;
Mass Screening;
Salmonella Infections;
Salmonella typhi*;
Salmonella*;
Sensitivity and Specificity;
Typhoid Fever
- From:Korean Journal of Clinical Microbiology
2007;10(2):123-127
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Widal test, whose sensitivity and specificity are variable from region to region depending on the incidence of typhoid fever, is still in use and shows a relatively low positive predictive value in Korea. We evaluated a rapid immunochromatographic assay (ICA) kit for the rapid diagnosis of Salmonella Typhi infection. METHODS: Twenty five patients with Salmonella Typhi bacteremia were selected for the patient group. For the control group, 21 nontyphoidal salmonellosis patients, 56 non-Salmonella bacteremia patients, and 57 healthy individuals were selected for evaluating the specificity of ICA test. Each serum specimen was tested with the SD Salmonella Typhi IgG/IgM kits (Standard Diagnostics, Inc., Yongin, Korea). RESULTS: The ICA showed a sensitivity of 100% in Salmonella Typhi patients, and specificities of 61.9%, 82.1% and 91.2% in nontyphoidal salmonellosis patients, non-Salmonella bacteremia patients, and healthy individuals, respectively. The ICA test showed a high sensitivity but a low specificity when compared with the Widal test. CONCLUSION: The ICA test by SD Salmonella Typhi IgG/IgM kit is highly sensitive, but its specificity is relatively low. The ICA test is simple, objective, and suitable for screening typhoid fever.
