MiR-372 regulates the proliferation, apoptosis and migration of 786-O cell by inhibiting PI3K/AKT/mTOR signaling pathway
10.3760/cma.j.cn115396-20231023-00099
- VernacularTitle:MiR-372通过抑制PI3K/AKT/mTOR信号通路调节786-O细胞的增殖、凋亡和迁移
- Author:
Xin WANG
1
;
Yu ZHANG
;
Shiqi JI
;
Qingjun LIU
Author Information
1. 首都医科大学附属北京地坛医院泌尿外科，北京 100102
- Keywords:
MicroRNAs;
Cell proliferation;
Cell apoptosis;
Cell migration assays;
MiR-372;
Clear cell renal carcinoma
- From:
International Journal of Surgery
2024;51(6):413-418
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether miR-372 can affect the proliferation, migration and apoptosis of renal clear cell carcinoma (ccRCC) cell under different expression conditions, and to study the effect of miR-372 on PI3K/AKT/mTOR signaling pathway.Methods:ccRCC cell line (786-O) and normal renal epithelial cell line (HK-2) were selected to detect the expression level of miR-372 in 786-O cell and HK-2 cell by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). The mimic group and negative control group were prepared by transfection with the mimic agent, and the inhibitor group and negative control group were prepared by transfection with the inhibitor. After stable transcription of cell lines was obtained, cell migration was detected by cell scratch assay, cell proliferation was detected by CCK8 method, and cell apoptosis was detected by flow cytometry, and the expression levels of proliferation and apoptosis related proteins and PI3K/AKT/mTOR signaling pathway-related proteins were detected by Western blotting. Measurement data were expressed as mean ± standard deviation ( ± s), and independent sample t-test was used for comparison between groups. Results:The expression level of miR-372 was decreased in 786-O cell. Overexpression of miR-372 inhibited the expression of proliferating cell nuclear antigen, promoted the expression of Bcl-2 and Caspase3, and reduced the phosphorylation level of PI3K/AKT/mTOR signaling pathway in 786-O cell.Conclusions:miR-372 can inhibit the migration and proliferation of 786-O cell, and promote cell apoptosis. In addition, miR-372 can inhibit the PI3K/AKT/mTOR signaling pathway.
