Expression level of LAMA2 in pan-cancer and its correlation with prognosis and immune microenvironment
10.3760/cma.j.cn115396-20230730-00014
- VernacularTitle:LAMA2在泛癌中的表达水平及其与预后、免疫微环境的相关性分析
- Author:
Yichuan WANG
1
;
Rui CAO
;
Zhenghao CHEN
;
Ye TIAN
Author Information
1. 首都医科大学附属北京友谊医院泌尿外科，北京 100050
- Keywords:
Computational biology;
Immunity;
Therapeutics;
LAMA2;
Tumor microenvironment
- From:
International Journal of Surgery
2024;51(6):376-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between the LAMA2 gene expression and the survival time of cancer patients, tumor microenvironment, tumor immune cell infiltration, and the immunotherapy responsiveness in pan-cancer. Methods:Download the expression data matrix and the clinical data from TCGA and IMvigor210, furthermore analyze the correlation between the expression level of LAMA2 and survival time, tumor microenvironment, and immunotherapy responsiveness by R and perform the gene set enrichment analysis of LAMA2 in pan-cancer to explore the related pathways. The Wilcoxon test was used to compare the expression difference of LAMA2 between tumor tissues and normal tissues. Kaplan-Meier method was used for survival analysis, and univariate COX regression analysis was used to evaluate the risk ratio relationship of LAMA2 in various tumors. Spearman correlation analysis was used to evaluate the correlation between the expression level of LAMA2 and tumor microenvironment immune score, as well as immune cell infiltration. Pearson correlation analysis was used to evaluate the correlation between the immunotherapy responsiveness and the expression level of LAMA2. Results:Compared with normal tissues, the expression level of LAMA2 was significantly decreased in most cancer types. In addition, the up-regulation or down-regulationand of LAMA2 expression may indicate the prognosis of patients. Univariate COX regression analysis showed that LAMA2 was significantly associated with prognosis in bladder cancer, renal papillary cell carcinoma, adrenocortical carcinoma, and so on. LAMA2 expression level was also significantly correlated with tumor microenvironment score, immune score and stromal score. Furthermore, the expression level of LAMA2 was correlated with the infiltration of tumor immune cells, including B cell, CD8 + T cell, regulatory T cell, mast cell, and so on. The results of gene set enrichment analysis show that the LAMA2 was mostly involved in the biological process of ion channels, gene expression, and skeletal muscle movement. LAMA2 expression was also associated with tumor mutation burden, microsatellite instability, and cell programmed death-ligand 1 immunotherapy responsiveness. Conclusions:LAMA2 has predictive value for survival and prognosis of patients in tumors, and is significantly correlated with tumor microenvironment, immune cell infiltration, tumor microenvironment, immunotherapy responsiveness, etc. As a potential tumor marker, LAMA2 provides a new direction for tumor prognosis judgment and treatment selection.
