Effect of PEG-PLGA co-loaded resveratrol nanoparticles on colon cancer cells through EMT and PI3K/Akt signaling pathways
10.3760/cma.j.cn121382-20240304-00507
- VernacularTitle:PEG-PLGA共载白藜芦醇纳米粒通过EMT和PI3K/Akt信号通路对结肠癌细胞的影响
- Author:
Hongliang GAO
1
;
Hao ZHANG
;
Mingzheng LI
Author Information
1. 天津市北辰医院外科，天津 300400
- Keywords:
Resveratrol;
Colon cancer;
Nanoparticles;
Epithelial-mesenchymal transition;
Phosphatidylinositol-3-kinase;
Protein kinase B
- From:
International Journal of Biomedical Engineering
2024;47(5):457-462
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of polyethylene glycol-poly (lactic-co-glycolic acid) (PEG-PLGA) co-loaded resveratrol nanoparticles on colon cancer cells through epithelial-mesenchymal transition (EMT) and phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) signaling pathways.Methods:Human colon cancer HCT116 cells were randomly divided into the control group and the experimental group. The experimental group was supplemented with 50 μl of 10 μmol/L PEG-PLGA co-loaded resveratrol nanoparticle solution, and the control group was supplemented with the same volume of medium without adding any treatment substance. Cell viability was determined using the MTT assay, apoptosis rate was analyzed by flow cytometry, and cell migration ability was detected by a scratch test. Western Blot was used to analyze the expression of proteins of related signaling pathways such as apoptosis, EMT, and PI3K/Akt.Results:Compared with the control group, the survival rate of colon cancer cells in the experimental group was reduced and the apoptosis rate was increased ( P < 0.05). Compared with the control group, the scratch width of HCT116 cells in the experimental group was greater ( P < 0.001). Compared with the control group, the expression of B-cell lymphoma-2 (Bcl-2) protein in the experimental group was down-regulated ( P < 0.01), while the expression of Bcl-2 associated X protein (Bax) was up-regulated ( P < 0.05). Compared with the control group, the expression of N-cadherin protein in the experimental group was down-regulated, while the expression of E-cadherin was up-regulated ( P < 0.01). Compared with the control group, the levels of p-PI3K and p-Akt in the experimental group were down-regulated (both P < 0.01). Conclusions:PEG-PLGA co-loaded resveratrol nanoparticles can reduce the cell viability, proliferation, and migration of colon cancer cells, which may be related to the inhibition of EMT and PI3K/Akt signaling pathways.
