Mechanism of extracts of Rhizoma Sparganii and Rhizoma Curcumae on cartilage damage and NOX2/ROS/NF-κB signaling pathway in osteoarthritis rats
10.3760/cma.j.cn121382-20240102-00306
- VernacularTitle:基于NOX2/ROS/NF-κB信号通路探讨三棱、莪术提取物对骨关节炎大鼠软骨细胞损伤的作用机制
- Author:
Jinchao MA
1
;
Minghao CHEN
;
Chunqian JIANG
;
Yang WANG
;
Xingguo LIU
;
Yongquan LI
Author Information
1. 天津市泰达医院骨科，天津 300457
- Keywords:
Osteoarthritis;
Rhizoma Sparganii extract;
Rhizoma Curcumae extract;
NADPH oxidase 2;
Reactive oxygen species;
Nuclear factor-κB;
Signaling pathwa
- From:
International Journal of Biomedical Engineering
2024;47(3):242-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of extracts of Rhizoma Sparganii and Rhizoma Curcumae on cartilage damage in osteoarthritis rats and NADPH oxidase 2 (NOX2)/reactive oxygen species (ROS)/nuclear factor-κB (NF-κB) signaling pathways. Methods:Rats (50 cases) were divided into the sham group, and model group, as well as the low, medium, and high dose groups of extracts of Rhizoma Sparganii and Rhizoma Curcumae, with 10 rats in each group. Except for sham group, the rat model of cartilage damage in knee osteoarthritis was established. On the second day after modeling, the rats in the low, medium, and high dose groups received intragastric extracts perfusion of Rhizoma Sparganii and Rhizoma Curcumae at the doses of 5, 10, and 20 g/kg respectively. The rats in the sham and model groups received intragastric equivalent 0.9% sodium chloride solution perfusion, once daily, for 20 days by continuous administration. The knee joint behavior, bone metabolism indicators, serum superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH) levels, inflammatory factors, NOX2, and NF-κB levels of each group were observed. Results:Compared with the model group, the behavioral abnormality scores, cartilage oligomeric matrix protein (COMP), MDA, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), NOX2, and NF-κB levels in the low, medium, and high dose groups were all gradually decreased (all P < 0.05), while proteoglycan, SOD, GSH, and interleukin-10 (IL-10) levels in the low, medium, and high dose groups were all gradually increased (all P < 0.05), and it was dose-dependent. Conclusions:Rhizoma Sparganii and Rhizoma Curcumae extracts can effectively improve cartilage damage in osteoarthritis rats, and it may be related to the inhibition of the NOX2/ROS/NF-κB signaling pathway.
