Frequency of CD36 Deficiency on Platelets and Monocytes in Koreans.
- Author:
Yousun CHUNG
1
;
Sang Mee HWANG
;
Myoung Shin KIM
;
Yun Ji HONG
;
Taek Soo KIM
;
Kyoung Un PARK
;
Junghan SONG
;
Kyou Sup HAN
Author Information
1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. m91w95@dreamwiz.com
- Publication Type:Original Article
- Keywords:
CD36;
Korean;
Platelet;
Monocyte
- MeSH:
Antibodies;
Blood Platelets;
Flow Cytometry;
Fluorescence;
Humans;
Isoantibodies;
Monocytes*;
Phenotype;
Platelet Transfusion;
Pregnancy;
Purpura;
Thrombocytopenia
- From:Korean Journal of Blood Transfusion
2014;25(1):28-33
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: CD36 deficiency was first identified in a patient who showed refractoriness to HLA-matched platelet transfusion. CD36 deficiency can be divided into two subgroups. The type I phenotype is characterized by platelets and monocytes exhibiting CD36 deficiency. The type II phenotype lacks surface expression of CD36 in platelets only. In this study, the frequency of type I and type II CD36 deficiency in Koreans was evaluated. METHODS: A total of 220 samples were randomly selected from subjects who requested CBC testing from August 2013 to February 2014. The expression levels of CD36 on platelets and monocytes were analyzed by flow cytometry using FITC-conjugated CD36 antibodies. Correlation between the median fluorescence intensity of CD36 and the number of platelets or monocytes was evaluated using Pearson's correlation coefficient. RESULTS: Type I phenotype, lacking CD36 on platelets and monocytes, was present in 0.9% and type II, lacking CD36 on platelets, was present in 3.2%. The median fluorescence intensity of CD36 did not show correlation with the count of platelets or monocytes. CONCLUSION: Type I subjects may produce alloantibodies against CD36 following transfusion or pregnancy, leading to refractoriness to HLA-matched platelet transfusion, post-transfusion purpura, or neonatal immune thrombocytopenia. Studies to determine exact frequency of CD36 deficiency in Koreans, including a larger population, should be conducted, and more case reports on patients immunized against CD36 are also needed in order to elucidate the clinical importance and relevance of CD36 deficiency testing and the transfusion of CD36-deficient platelets.
