Molecular epidemiological characteristics and risk factors of HvKp infection in Hulunbuir area
10.3969/j.issn.1673-4130.2024.20.003
- VernacularTitle:呼伦贝尔地区HvKp分子流行病学特征及其感染的危险因素分析
- Author:
Yuan TIAN
1
;
Wanli GAO
;
Hui SUN
;
Xianwei LIU
;
Shihua ZHANG
;
Gang SUN
Author Information
1. 内蒙古民族大学附属医院医学检验中心,内蒙古通辽 028000
- Keywords:
hypervirulent Klebsiella pneumoniae;
molecular epidemiology;
risk factors;
Hulunbuir region
- From:
International Journal of Laboratory Medicine
2024;45(20):2443-2448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular epidemiology of hypervirulent Klebsiella pneumoniae(HvKp)and its independent risk factors for infection,and to provide research basis for anti-infection treat-ment.Methods A total of 519 Klebsiella pneumoniae strains were collected from Inner Mongolia Forestry General Hospital from January 2020 to December 2022.String test was used to distinguish hypermyxoid strains(HMV-Kp)and non-HMV-KP.The rmpA,rmpA2,and iutA genes were detected by common PCR agarose gel electrophoresis to screen the HvKp strains.Multilocus sequence typing analysis was performed on 60 HvKp strains and the minimum spanning tree was drawn.Multivariate Logistic regression was used to ana-lyze the risk factors of HvKp infection.Results The positive rate of HMV-Kp was 39.69％,the positive rate of HvKp was 37.19％,and HMV-Kp accounted for 76.68％of HvKp.The detection rate of HvKp in general surgery department was the highest,and the detection rate of HvKp in pus specimens was the highest.By ST typing comparison,a total of 18 types of 60 HvKp strains were detected,ST23 type was the most common type(50.00％),followed by ST86 type(8.33％).Multivariate Logistic regression analysis showed that male,liver abscess,infection or suppuration of other tissues and organs,and use of macrolide antibiotics in the past 3 months were independent risk factors for HvKp infection(P＜0.05).Conclusion There is a strong associa-tion between HvKp and HMV-Kp strains,and ST23 type is the dominant type in this study.Male,liver ab-scess,infection or suppuration of other tissues and organs,and use of macrolide antibiotics in the past 3 months are independent risk factors for HvKp infection.
