Relationship between CENPA and GRHL2 and clinicopathological characteristics of breast cancer and its clinical value in prognosis evaluation
10.3969/j.issn.1673-4130.2024.19.003
- VernacularTitle:CENPA、GRHL2与乳腺癌临床病理特征的关系及其在预后评估中的临床价值
- Author:
Jingjing WANG
1
;
Suqin SHEN
Author Information
1. 南京医科大学附属妇产医院/南京市妇幼保健院检验科,江苏南京 210004
- Keywords:
breast cancer;
centromere protein A;
granular head like 2;
prediction model
- From:
International Journal of Laboratory Medicine
2024;45(19):2316-2322
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of centromere protein A(CENPA)and granular head like 2(GRHL2)in breast cancer tissues,construct a prognostic model of breast cancer and analyze its clinical application value.Methods The expression of CENPA and GRHL2 in 1 085 breast cancer tissues and 291 con-trol tissues were analyzed using gene expression profiling interactive analysis(GEPIA)database.The relation-ship between CENPA and GRHL2 expression and the survival of breast cancer patients was analyzed using Kaplan-Meier Plotter online website.A total of 84 patients with primary unilateral breast cancer treated in the hospital from January 2018 to June 2020 were selected as the research objects.The expression of CENPA and GRHL2 was detected by immunohistochemistry,and the relationship between CENPA and GRHL2 and clini-copathological characteristics of breast cancer was analyzed.Spearman correlation analysis was used to analyze the correlation between CENPA,GRHL2 and carbohydrate antigen 153(CA153).Receiver operating charac-teristic(ROC)curve was used to analyze the prognostic value of CENPA,GRHL2 and CA153 in breast canc-er.Cox proportional hazards regression model was used to analyze the risk factors of breast cancer prognosis.Results GEPIA database showed that the expression levels of CENPA and GRHL2 in breast cancer tissues were higher than those in normal tissues(P＜0.05).Kaplan-Meier Plotter analysis showed that the expres-sion levels of CENPA and GRHL2 increased,and the survival rate of patients decreased(Log-rank P＜0.001).The expressions of CENPA and GRHL2 were positively correlated with CA153(r=0.410,0.602,P＜0.001).The area under the curve of CENPA,GRHL2 and CA153 in breast cancer diagnosis was 0.878.The expressions of CENPA and GRHL2 were correlated with TNM stage,histological grade,lymph node me-tastasis and epidermal growth factor receptor-2 expression(P＜0.05).Cox regression analysis showed that TNM stage Ⅲ.histological grade Ⅲ,lymph node metastasis and high expression of CENPA and GRHL2 were independent prognostic risk factors of breast cancer(P＜0.05).Conclusion CENPA and GRHL2 are valua-ble biomarkers for the prognosis evaluation of breast cancer.Combined detection of CENPA and GRHL2 with CA153 has a high predictive value for the prognosis of breast cancer.
