Analysis of bone marrow morphological characteristics in acute myeloid leukemia with positive RUNX1-RUNX1T1 fusion gene
10.3969/j.issn.1673-4130.2024.17.007
- VernacularTitle:RUNX1-RUNX1T1融合基因阳性急性髓系白血病骨髓形态学特点分析
- Author:
Mengna LIU
1
;
Hongling WANG
;
Huanjin LIAO
;
Li LI
;
Ping BAI
Author Information
1. 上海交通大学医学院附属第一人民医院检验医学中心,上海 200080
- Keywords:
RUNX1-RUNX1T1;
fusion gene;
acute myeloid leukemia;
bone marrow morphology
- From:
International Journal of Laboratory Medicine
2024;45(17):2081-2086
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the morphological characteristics of bone marrow in RUNX1-RUNX1T1 fusion gene-positive AML.Methods From January 2017 to January 2023,20 cases of RUNX1-RUNX1T1 fusion gene-positive AML patients newly diagnosed in this hospital were selected as group A,20 cases of AML patients without fusion gene were selected as group B,and 24 cases of PML-RARA fusion gene-positive AML patients were selected as group C.Results of bone marrow morphology,immunology,cytogenet-ics and molecular biology were analyzed retrospectively.The morphological characteristics in similar cases combined with domestic and foreign literature were summarized.Results The number of promyelocytes in group A increased in different degrees,the difference was statistically significant compared with group B(P＜0.05),and the difference was also statistically significant compared with group C(P＜0.001).In group A,some promyelocytes had abnormal morphology,and the mRNA level of fusion gene was positively correlated with the number of promyelocytes(r=0.478,P=0.039).Patients with expressions of CD56,CD19 and c-Kit mutation in group A was much more than that in group B(P＜0.05).No significant difference was detected in remission rate after the first course of chemotherapy between group A and group B(P＞0.05).Besides,the simultaneous expression of CD56 and CD19 in group A was easy to accompany the loss of sex chromosome.Conclusion AML with positive RUNX1-RUNX1T1 fusion gene could be identified with bone marrow mor-phology,immunology,cytogenetics and molecular biology.Granulocyte in the bone marrow is prone to differ-ent degrees of pathological hematopoiesis with great variability.The increased number and abnormal morphol-ogy of promyelocytes in bone marrow may be one of the morphologic features of AML with positive RUNX1-RUNX1T1 fusion gene.
