Expression of serum miR-126 and miR-132 in patients with sepsis complicated with ARDS and their relationship with disease outcome
10.3969/j.issn.1673-4130.2024.16.004
- VernacularTitle:脓毒症并发ARDS患者血清miR-126、miR-132表达及其与疾病转归的关系
- Author:
Mengdi WU
1
;
Sheng DING
Author Information
1. 连云港市第一人民医院急诊内科,江苏连云港 222000
- Keywords:
sepsis;
acute respiratory distress syndrome;
disease outcome
- From:
International Journal of Laboratory Medicine
2024;45(16):1936-1940
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of serum microRNA(miR)-126 and miR-132 in patients with sepsis complicated with acute respiratory distress syndrome(ARDS)and their relationship with disease outcome.Methods A total of 106 patients with sepsis complicated with ARDS admitted to a hospital from January 2021 to July 2023 were selected as the study group,another 110 patients with simple sepsis in the hos-pital in the same period were selected as the control group.Real-time fluorescence quantitative PCR was used to detect the relative expression levels of miR-126 and miR-132 in serum of all subjects.106 patients with sep-sis complicated with ARDS were divided into survival group(n=65)and death group(n=41)according to the prognosis of the disease after 28 days'treatment.The relative expression levels of miR-126 and miR-132 in serum of all groups were compared.Receiver operating characteristic curve was drawn to analyze the prognos-tic value of serum miR-126 and miR-132 in patients with sepsis complicated with ARDS.Multivariate Logistic stepwise regression was used to analyze the risk factors of disease outcome in patients with sepsis complicated with ARDS.Results The relative expression levels of miR-126 and miR-132 in the study group were lower than those in the control group,and the difference was statistically significant(P＜0.05).The relative expres-sion levels of serum miR-126 and miR-132 in the death group were lower than those in the survival group,and the difference was statistically significant(P＜0.05).The area under the curve(AUC)of serum miR-126 and miR-132 to predict the disease outcome of patients with sepsis complicated with ARDS were 0.749(95％CI:0.705-0.818)and 0.825(95％CI:0.782-0.875)respectively,and the combined forecast AUC was 0.908(95％CI:0.859-0.954).Multivariate Logistic stepwise regression showed that multiple organ failure(OR=3.494,95％CI:1.519-8.037),miR-126＜0.75(OR=4.707,95％CI:1.834-12.083),miR-132＜7.73(OR=5.307,95％CI:2.104-13.385)were risk factors for disease outcome in patients with sepsis complicated with ARDS(P＜0.05).Conclusion The decreased expression of serum miR-126 and miR-132 in sepsis complicated with ARDS is related to the adverse disease outcome,and the two may be used as biological indicators to eval-uate the prognosis of sepsis complicated with ARDS.
