Comprehensive analysis of mRNA/miRNA networks in peripheral blood of patients with schizophrenia
10.3969/j.issn.1673-4130.2024.15.014
- VernacularTitle:精神分裂症患者外周血中mRNA/miRNA网络综合分析
- Author:
Dongdong QI
1
;
Shifa MA
;
Xiaolong LI
;
Kegang WANG
Author Information
1. 呼伦贝尔市第三人民医院/呼伦贝尔市精神卫生中心精神疾病基础与临床实验室,内蒙古牙克石 022150
- Keywords:
schizophrenia;
mRNA/microRNA networks;
bioinformatics
- From:
International Journal of Laboratory Medicine
2024;45(15):1867-1873
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the comprehensive analysis of mRNA/microRNA(miRNA)networks in peripheral blood of patients with schizophrenia.Methods A total of 6 schizophrenic patients treated in a hos-pital from December 2022 to February 2023 were included as the experimental group and 4 volunteers as the control group.Blood samples of the two groups were collected and the mRNA and miRNA expression levels were detected by RNA-seq technique.Finally,the gene set GSE145554 in Gene Expression Omnibus was used for bioinformatics verification analysis.Results Compared with the control group,a total of 2 133 mRNA differences were detected in the experimental group,including 1 410 mRNA up-regulated and 723 mRNA down-regulated.There were 150 differential miRNAs,including 72 up-regulated miRNAs and 78 down-regula-ted miRNAs.Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment biological processes and signaling pathways mainly include mammalian target of rapamycin(mTOR)signaling pathway,phos-phatidylinositide 3-kinases(PI3K)-serine/threonine-protein kinase(AKT)signaling pathway,necroptosis,and protein processing in the endoplasmic reticulum endoplasmic reticulum signaling pathways etc.Conclusion PI3K-AKT,mTOR,necroptosis and protein processing in endoplasmic reticulum signaling pathways are in-volved in the occurrence and development of schizophrenia,and provide a new research direction for the pre-vention,diagnosis and treatment of schizophrenia.
