Serum miR-125a-5p,miR-127-3p expression and clinical significance in patients with hepatitis B virus-associated hepatocellular carcinoma
10.3969/j.issn.1673-4130.2024.15.013
- VernacularTitle:乙型肝炎病毒相关性肝细胞肝癌患者血清miR-125a-5p、miR-127-3p表达及临床意义
- Author:
Jixu WANG
1
;
Guodong ZHANG
;
Maoli CHEN
;
Yongfeng HAN
Author Information
1. 山东第一医科大学附属人民医院感染科,山东济南 271199
- Keywords:
hepatitis B virus-associated hepatocellular carcinoma;
microRNA-125a-5p;
microRNA-127-3p;
pathological features;
prognosis
- From:
International Journal of Laboratory Medicine
2024;45(15):1860-1866
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the serum microRNA(miR)-125a-5p,miR-127-3p expression and clinical significance in patients with hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC).Meth-ods A total of 90 HBV-associated HCC patients admitted to a hospital from January 2018 to October 2020 were selected as the HBV-associated HCC group.Another 90 healthy subjects from the same period were se-lected as the control group.Serum miR-125a-5p and miR-127-3p expressions were detected by real-time fluo-rescence quantitative polymerase chain reaction.To analyze the relationship between miR-125a-5p and miR-127-3p expression and pathological features of patients with HBV-associated HCC.Patients with HBV-associ-ated HCC were divided into high and low expression groups according to the mean values of serum miR-125a-5p and miR-127-3p expression,and the different survival curves of each groups were plotted by the Kaplan-Meier method.Factors affecting the prognosis of patients with HBV-associated HCC were analyzed by Cox re-gression,and the predictive value of serum miR-125a-5p and miR-127-3p expression on the death of patients with HBV-associated HCC was analyzed by receiver operating characteristic(ROC)curve.Results The rela-tive expression levels of miR-125a-5p and miR-127-3p in HBV-associated HCC group were lower than those in control group,with statistical significance(P＜0.05).There were significant differences in the expression of miR-125a-5p and miR-127-3p in serum of HBV-associated HCC patients with different tumor size,differentia-tion degree,vascular invasion,TNM stage and lymph node metastasis(P＜0.05).Kaplan-Meier survival curve analysis showed that the 3-year overall survival rate(64.58％)of the group with high expression of miR-125a-5p was higher than that of the group with low expression of miR-125a-5p(38.10％).The 3-year overall survival rate of miR-127-3p high expression group(66.00％)was higher than that of miR-127-3p low expres-sion group(35.00％),and the difference was statistically significant(x2=7.770,9.507,P=0.005,0.002).The independent risk factors for death in HBV-associated HCC patients were maximum tumor diameter ≥5 cm,low differentiation,vascular invasion,TNM stage Ⅲ-Ⅳ,and lymph node metastasis,and the independent protective factors were elevated miR-125a-5p and miR-127-3p.ROC curve analysis results showed that the are-a under the curve(AUC)for the combined prediction of serum miR-125a-5p and miR-127-3p expression was 0.907,which was significantly higher than the AUC for the individual prediction of serum miR-125a-5p and miR-127-3p expression(0.790,0.787),with a statistically significant difference(Z=2.691,3.152,P=0.007,0.002).Conclusion The low expression of serum miR-125a-5p and miR-127-3p in HBV-associated HCC pa-tients is related to tumor maximum diameter,differentiation degree,vascular invasion,TNM stage and prog-nosis.The combined expression of serum miR-125a-5p and miR-127-3p has a high predictive value for the prognosis of HBV-associated HCC patients.
