Progress in the application of digital PCR for the detection of Mycobacterium tuberculosis
10.3969/j.issn.1673-4130.2024.14.018
- VernacularTitle:数字PCR技术检测结核分枝杆菌的应用进展
- Author:
Yiting LIN
1
;
Chunmei ZHOU
;
Wei GUO
Author Information
1. 上海市老年医学中心检验科,上海 201100
- Keywords:
digital polymerase chain reaction;
tuberculosis;
Mycobacterium tuberculosis;
drug re-sistance genes
- From:
International Journal of Laboratory Medicine
2024;45(14):1756-1760,1766
- CountryChina
- Language:Chinese
-
Abstract:
Tuberculosis is a worldwide chronic infectious disease that has become a serious threat to human health.Early diagnosis,drug resistance screening and control of disease transmission are key aspects of TB prevention and treatment.However,existing diagnostic techniques and drug sensitivity tests are time-consu-ming and difficult to realize the purpose of early diagnosis and drug resistance screening,which greatly limits the control of disease transmission.Digital polymerase chain reaction(dPCR)is the third generation of poly-merase chain reaction(PCR),which is a quantitative nucleic acid assay with high sensitivity and no calibration curve.This article provides an overview of the principles of dPCR and its application in tuberculosis diagnosis,drug resistance screening and transmission monitoring,compares dPCR with other tuberculosis detection methods,and looks at the challenges and future prospects of dPCR in clinical tuberculosis laboratory applica-tions.
