Relationship between serum miR-130b-3p and miR-181a-5p expression and pregnancy outcome in patients with gestational diabetes mellitus
10.3969/j.issn.1673-4130.2024.13.018
- VernacularTitle:妊娠糖尿病患者血清miR-130b-3p、miR-181a-5p表达与妊娠结局的关系
- Author:
Kun XING
1
;
Tongqing ZHANG
;
Cheng KANG
Author Information
1. 北京航天总医院妇产科,北京 100071
- Keywords:
gestational diabetes mellitus;
miR-130b-3p;
miR-181a-5p;
pregnancy outcome
- From:
International Journal of Laboratory Medicine
2024;45(13):1627-1631
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between serum microRNA(miRNA)miR-130b-3p and miR-181a-5p expression and pregnancy outcome in patients with gestational diabetes mellitus.Methods From June 2020 to August 2022,a total of 124 pregnant women with gestational diabetes mellitus diagnosed and treated in Beijing Aerospace General Hospital and Beijing Obstetrics and Gynecology Hospital were selected as the study group.According to the pregnancy outcome,they were separated into a good group and an adverse group.Real-time fluorescence quantitative PCR method was applied to detect the expression of miR-130b-3p and miR-181a-5p in serum.Pearson method was applied to analyze serum miR-130b-3p,miR-181a-5p,and their correlation with blood lipids and blood glucose.Logistic regression was used to analyze the influencing factors of adverse pregnancy outcomes in patients with gestational diabetes mellitus.Results The levels of total cho-lesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),fasting blood glucose(FBG),fasting insulin(FINS),insulin resistance index(HOMA-IR),and the relative expression level of miR-130b-3p in the study group were higher than those in the control group(P<0.05).The relative expression level of miR-181a-5p was reduced compared to the control group(P<0.05).The levels of TC,TG,LDL-C,FBG,FINS,HOMA-IR and miR-130b-3p in the adverse group were higher than those in the good group(P<0.05),while the relative expression level of miR-181a-5p was lower than that in the good group(P<0.05).According to Pearson correlation analysis,serum miR-130b-3p was negatively correlated with miR-181a-5p(P<0.05),serum miR-130b-3p was positively correlated with TC,TG,LDL-C,FBG,FINS,HOMA-IR(P<0.05),and miR-181a-5p was negatively correlated with TC,TG,LDL-C,FBG,FINS,HOMA-IR(P<0.05).Logistic regression analysis showed that high levels of miR-130b-3p,TC,TG,LDL-C,FBG,FINS and low rel-ative expression level of miR-181a-5p were risk factors for adverse pregnancy outcomes of gestational diabetes mellitus(P<0.05).Conclusion The serum miR-130b-3p relative expression level in patients with gestational diabetes mellitus is increased and the serum miR-181a-5p relative expression level is decreased,both of which are closely related to the pregnancy outcome.
