Single-cell transcriptomics reveals cell atlas and identifies cycling tumor cells responsible for recurrence in ameloblastoma
- Author:
Xiong GAN
1
;
Xie NAN
;
Nie MIN
;
Ling RONGSONG
;
Yun BOKAI
;
Xie JIAXIANG
;
Ren LINLIN
;
Huang YAQI
;
Wang WENJIN
;
Yi CHEN
;
Zhang MING
;
Xu XIUYUN
;
Zhang CAIHUA
;
Zou BIN
;
Zhang LEITAO
;
Liu XIQIANG
;
Huang HONGZHANG
;
Chen DEMENG
;
Cao WEI
;
Wang CHENG
Author Information
- From: International Journal of Oral Science 2024;16(2):251-264
- CountryChina
- Language:Chinese
- Abstract: Ameloblastoma is a benign tumor characterized by locally invasive phenotypes,leading to facial bone destruction and a high recurrence rate.However,the mechanisms governing tumor initiation and recurrence are poorly understood.Here,we uncovered cellular landscapes and mechanisms that underlie tumor recurrence in ameloblastoma at single-cell resolution.Our results revealed that ameloblastoma exhibits five tumor subpopulations varying with respect to immune response(IR),bone remodeling(BR),tooth development(TD),epithelial development(ED),and cell cycle(CC)signatures.Of note,we found that CC ameloblastoma cells were endowed with stemness and contributed to tumor recurrence,which was dominated by the EZH2-mediated program.Targeting EZH2 effectively eliminated CC ameloblastoma cells and inhibited tumor growth in ameloblastoma patient-derived organoids.These data described the tumor subpopulation and clarified the identity,function,and regulatory mechanism of CC ameloblastoma cells,providing a potential therapeutic target for ameloblastoma.
