Claudin-1, -2, -4, and -5: comparison of expression levels and distribution in equine tissues.
10.4142/jvs.2016.17.4.445
- Author:
Bonn LEE
1
;
Hee Young KANG
;
Dong Oh LEE
;
Changhwan AHN
;
Eui Bae JEUNG
Author Information
1. Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea. ebjeung@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Equus ferus caballus;
claudins;
paracellular transportation;
tight junctions
- MeSH:
Animals;
Architectural Accessibility;
Blotting, Western;
Claudin-1*;
Claudin-2;
Claudin-4;
Claudin-5;
Claudins;
Colic;
Duodenum;
Female;
Horse Diseases;
Horses;
Immunohistochemistry;
Kidney;
Liver;
Lung;
Methods;
Organ Specificity;
Ovary;
Tight Junctions;
Transportation;
Uterus
- From:Journal of Veterinary Science
2016;17(4):445-451
- CountryRepublic of Korea
- Language:English
-
Abstract:
Claudins, which are known as transmembrane proteins play an essential role in tight junctions (TJs) to form physical barriers and regulate paracellular transportation. To understand equine diseases, it is helpful to measure the tissue-specific expression of TJs in horses. Major equine diseases such as colic and West Nile cause damage to TJs. In this study, the expression level and distribution of claudin-1, -2, -4, and -5 in eight tissues were assessed by Western blotting and immunohistochemistry methods. Claudin-1 was primarily identified in the lung, duodenum, and uterus, claudin-2 was evenly observed in equine tissues, claudin-4 was abundantly detected in the liver, kidney and uterus, and claudin-5 was strongly expressed in the lung, duodenum, ovary, and uterus, as determined by Western blotting method. The localization of equine claudins was observed by immunohistochemistry methods. These findings provide knowledge regarding the expression patterns and localization of equine claudins, as well as valuable information to understand tight junction-related diseases according to tissue specificity and function of claudins in horses.
