Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin.
10.4142/jvs.2016.17.4.459
- Author:
Rafael Herrera ALVAREZ
1
;
Fabio Luis Nogueira NATAL
;
Maria Teresa Carvalho Pinto RIBELA
;
Beatriz Elane DE ALMEIDA
;
João Ezequiel DE OLIVEIRA
;
Paolo BARTOLINI
Author Information
1. Agency for Agribusiness Technology of São Paulo, 13400-970 Piracicaba, Brazil. rherrera@apta.sp.gov.br
- Publication Type:Original Article
- Keywords:
equine chorionic gonadotropin;
gilts;
high pressure liquid chromatography;
ovarian response;
rats
- MeSH:
Animals;
Animals, Domestic;
Biological Assay;
Chorion*;
Chorionic Gonadotropin*;
Chromatography, High Pressure Liquid;
Electrocardiography;
Female;
Humans;
Ovulation Induction;
Rats;
Weight Gain;
World Health Organization
- From:Journal of Veterinary Science
2016;17(4):459-466
- CountryRepublic of Korea
- Language:English
-
Abstract:
Ovarian stimulation with commercial preparations of equine chorionic gonadotropin (eCG) produces extremely variable responses in domestic animals, ranging from excessive stimulation to practically no stimulation, when applied on the basis of their declared unitage. This study was conducted to analyze four commercial preparations from different manufacturers via reversed-phase HPLC (RP-HPLC) in comparison with a reference preparation and an official International Standard from the World Health Organization. The peaks obtained by this qualitative and quantitative physical–chemical analysis were compared using an in vivo bioassay based on the ovarian weight gain of prepubertal female rats. The RP-HPLC data showed one or two peaks close to a main peak (t(R) = 27.9 min), which were related to the in vivo bioactivity. Commercial preparations that have this altered peak showed very little or no in vivo activity, as demonstrated by rat ovarian weight and in peripubertal gilts induced to ovulate. Overall, these findings indicate that RP-HPLC can be a rapid and reliable tool to reveal changes in the physicochemical profile of commercial eCG that is apparently related to decreased biological activity of this hormone.
