Role of saliva proteinase 3 in dental caries
10.1038/ijos.2015.8;published29May2015
- Author:
Yang TENG-YU
1
;
Zhou WEN-JIE
;
Du YUE
;
Wu SONG-TAO
;
Yuan WEN-WEN
;
Yu YU
;
Su LIN
;
Luo YANG
;
Zhang JIE-HUA
;
Lu WAN-LU
;
Wang XIAO-QIAN
;
Chen JIAO
;
Feng YUN
;
Zhou XUE-DONG
;
Zhang PING
Author Information
1. State Key Laboratory of 0ral Diseases
- Keywords:
salivary;
caries;
proteinase 3;
pH;
Streptococcus mutans
- From:
International Journal of Oral Science
2015;(3):174-178
- CountryChina
- Language:Chinese
-
Abstract:
Salivary analysis can be used to assess the severity of caries. Of the known salivary proteins, a paucity of information exists concerning the role of proteinase 3 (PR3), a serine protease of the chymotrypsin family, in dental caries.Whole, unstimulated saliva was collected from children with varying degrees of active caries and tested using a Human Protease Array Kit and an enzyme-linked immunosorbent assay. Asignificantly decreased concentration of salivaryPR3was notedwith increasing severity of dental caries (P,0.01); a positive correlation (r50.87; P,0.01; Pearson’s correlation analysis) was also observed between salivary pHand PR3 concentration. In an antibacterial test, a PR3 concentration of 250 ng?mL21 or higher significantly inhibited Streptococcus mutans UA159 growth after 12 h of incubation (P,0.05). These studies indicate that PR3 is a salivary factor associated with the severity of dental caries, as suggested by the negative relationship between salivary PR3 concentration and the severity of caries as well as the susceptibility of S. mutans to PR3.
