Establishment and Validation of a Multiplex PCR Detection System for the Identification of Six Common Edible Meat Components
10.12116/j.issn.1004-5619.2023.531002
- VernacularTitle:鉴别6种常见食用肉类的多重PCR检测体系的构建及验证
- Author:
Zhi-Wei JIANG
1
,
2
;
Ruo-Cheng XIA
;
Rui-Yang TAO
;
Cheng-Tao LI
Author Information
1. 温州医科大学基础医学院法医学系,浙江 温州 325035
2. 司法鉴定科学研究院 上海市法医学重点实验室 司法部司法鉴定重点实验室 上海市司法鉴定专业技术服务平台,上海 200063
- Keywords:
forensic genetics;
mitochondrial DNA;
multiplex PCR;
species identification;
edible meat
- From:
Journal of Forensic Medicine
2024;40(3):254-260
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid,accurate,and sensitive multiplex PCR detection method for the simultaneous identification of the six common edible meats (beef,lamp,chicken,pork,goose,duck),and to evaluate its application value in meat adulteration identification.Methods Based on complete mitochondrial genomic sequences of six species in the GenBank database,DNA sequences (cattle:16S rRNA;sheep:COX-1;chickens:Cytb;pig:COX-1;goose:NADH2;duck:16S rRNA) with intra-species conservation and inter-species specificity were screened,and species-specific primers were designed to construct a multiplex PCR detection system that can simultaneously detect the meat of six common species.The species specificity,sensitivity and reproducibility of the system were studied,and the simulated mixture sample detection was performed.Results This study successfully constructed a multiplex PCR detection system that can detect the meats of six common species simultaneously.The system was not effective in DNA amplification of non-target species.When the DNA template sizes were 0.0625-2 ng/μL,the amplified products of all six species could be detected.The duck compo-nent was still detected when the mixing ratio of duck and beef was as low as 0.5%.Conclusion This study constructs and establishes a multiplex PCR detection system with strong specificity,high sensi-tivity,and good reproducibility.It can accurately identify the components of animal origin in common edible meats and provide a simple and practical method for identifying adulteration of common edible meats and meat products in China.
