Urine Metabolites Changes in Acute Myocardial Infarction Rats via Metabolomic Analysis
10.12116/j.issn.1004-5619.2023.431108
- VernacularTitle:基于代谢组学分析急性心肌梗死大鼠尿液代谢物变化
- Author:
Nian-Nian CHEN
1
;
Jiao-Fang YU
;
Peng WU
;
Li LUO
;
Ya-Qin BAI
;
Li-Kai WANG
;
Xiao-Qian LI
;
Zhan-Peng LI
;
Cai-Rong GAO
;
Xiang-Jie GUO
Author Information
1. 山西医科大学法医学院,山西 晋中 030600
- Keywords:
forensic pathology;
non-targeted metabolomics;
acute myocardial infarction;
ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS);
rats;
urine
- From:
Journal of Forensic Medicine
2024;40(3):227-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen biomarkers for forensic identification of acute myocardial infarction (AMI) by non-targeted metabolomic studies on changes of urine metabolites in rats with AMI.Methods The rat models of the sham surgery group,AMI group and hyperlipidemia+acute myocardial infarction (HAMI) group were established.Ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) was used to analyze the changes of urine metabolic spectrometry in AMI rats.Principal compo-nent analysis,partial least squares-discriminant analysis,and orthogonal partial least squares-discriminant analysis were used to screen differential metabolites.The MetaboAnalyst database was used to analyze the metabolic pathway enrichment and access the predictive ability of differential metabolites.Results A total of 40 and 61 differential metabolites associated with AMI and HAMI were screened,respec-tively.Among them,22 metabolites were common in both rat models.These small metabolites were mainly concentrated in the niacin and nicotinamide metabolic pathways.Within the 95% confidence in-terval,the area under the curve (AUC) values of receiver operator characteristic curve for N8-acetyl-spermidine,3-methylhistamine,and thymine were greater than 0.95.Conclusion N8-acetylspermidine,3-methylhistamine,and thymine can be used as potential biomarkers for AMI diagnosis,and abnormal metabolism in niacin and nicotinamide may be the main causes of AMI.This study can provide reference for the mechanism and causes of AMI identification.
