A New Multifunctional Biosensor Based on a Hairpin Oligonucleotide Probe and G-Quadruplex/Hemin DNAzyme
10.19756/j.issn.0253-3820.210896
- VernacularTitle:基于新型发夹核酸探针和G-四链体/血红素脱氧核酶的多功能生物传感器
- Author:
Lei WANG
1
;
Ji-Xiang SONG
;
Xing-Feng LIU
;
Yan-Qin HUANG
;
Qu-Li FAN
Author Information
1. 南京邮电大学,有机电子与信息显示国家重点实验室,江苏省生物传感材料与技术重点实验室,信息材料与纳米技术研究院,南京 210023
- Keywords:
G-Quadruplex;
DNAzyme;
Hemin;
Hemoglobin;
Biosensor
- From:
Chinese Journal of Analytical Chemistry
2024;52(8):1123-1131
- CountryChina
- Language:Chinese
-
Abstract:
A new multifunctional fluorescent"turn-on"biosensor was constructed based on a guanine-rich hairpin probe and G-quadruplex/hemin DNAzyme.The probe was labeled with quencher and fluorescein at 5′and 3′terminus,respectively.In the absence of the target,the probe form hairpin structure and the fluorescence of the fluorescein was quenched by the quencher.However,in the presence of the target,the hairpin structure transformed to G-quadruplex and the fluorescence was recovered.By monitoring the fluorescence of the probe before and after combining with the target,both hemin and hemoglobin were detected rapidly and conveniently with high sensitivity.The limit of detection for hemin and hemoglobin were estimated to be 283 pmol/L and 109 pmol/L,respectively.The specific interaction between the DNA probe and hemin were investigated via absorption and fluorescence spectra.Furthermore,L-cysteine was detected with naked eyes based on the peroxidase-like activity of the new G-quadruplex/hemin DNAzyme.The limit of detection for L-cysteine was estimated to be 3.6 μmol/L,which was lower than the normal level of intracellular L-cysteine(30-200 μmol/L).The method developed here was rapid,simple and sensitive for detection of hemin,hemoglobin and L-cysteine,demonstrating great potential in bioanalysis,drug screening,and disease diagnosis.
