Quantitative Detection of Procalcitonin in Blood by Nanozyme-based Lateral Flow Immunoassay
10.19756/j.issn.0253-3820.241059
- VernacularTitle:纳米酶增强免疫层析法定量检测血液中的降钙素原
- Author:
Yue ZHENG
1
;
Tong LIN
;
Yong-Hua XIONG
;
Meng-Shuo XU
;
Xi-Luan YAN
;
Xu-Jing GUO
;
Lei YANG
;
Liang GUO
Author Information
1. 南昌大学,食品科学与资源挖掘全国重点实验室,南昌 330047
- Keywords:
Lateral flow immunoassay;
Procalcitonin;
Rapid detection;
Nanozyme
- From:
Chinese Journal of Analytical Chemistry
2024;52(8):1082-1093
- CountryChina
- Language:Chinese
-
Abstract:
A rapid quantitative immunochromatographic assay for procalcitonin(PCT)using metal-organic frameworks modified with gold and platinum nanoparticles(MAPs)as labels was established in this work.The detection probe was prepared by conjugating MAPs with anti-PCT monoclonal antibody via an electrostatic adsorption method.Anti-PCT polyclonal antibody and sheep anti-mouse IgG were sprayed onto the nitrocellulose(NC)membrane as the test line and quality control line,respectively,to construct immunochromatographic strip for PCT quantitative detection via signal-amplification-based sandwich immunoassay.The results showed that the MAP-based immunochromatographic test had high sensitivity,high specificity,and good stability.The dynamic range for detection of PCT was 0.61 pg/mL-320 ng/mL,the detection limit was 0.25 pg/mL,and the intra-day and inter-day precision(Relative standard deviation)were less than 15%.The results of real sample analysis showed that a quite low volume of sample was required for detection of PCT in whole blood,which was of great significance for the early diagnosis,monitoring and treatment,and prognosis of inflammation.
