Performance Expansion and Optimization of Live Cell-based Arsenic Ion Microbial Sensors
10.19756/j.issn.0253-3820.231367
- VernacularTitle:基于活细胞的微生物砷离子传感器的性能拓展与优化
- Author:
Ming-Hang CUI
1
,
2
;
Jia-Jia LI
;
Jin WANG
;
Er-Kang WANG
;
Xiao-Na FANG
Author Information
1. 中国科学院长春应用化学研究所,电分析化学国家重点实验室,长春130022
2. 中国科学技术大学应用化学与工程学院,合肥230026
- Keywords:
Arsenic ion microbial biosensor;
Culture medium;
Growth phases of bacteria;
Fluorescent proteins reporter;
Response time
- From:
Chinese Journal of Analytical Chemistry
2024;52(6):885-892,中插38-中插43
- CountryChina
- Language:Chinese
-
Abstract:
During the development of whole-cell microbial sensors,factors such as cellular metabolic activity and signal output modes play pivotal roles in the stability and repeatability of the sensors,presenting numerous challenges for the standardization of sensor applications. This research focused on the arsenic ion sensor based on the RepL amplifier,adjusting the reporter genes,culture media,growth stages,and induction times of arsenic ions,aiming to investigate how these factors affect the sensor's detection performance. The results indicated that the cell's culturing environment,growth status (e.g.,different growth phases),type of reporter,and induction time all had significant impacts on the performance of the arsenic ion sensor. First,the stability of the sensors varied greatly in different media,all the three sensors displayed greater stability in LB culture medium. Meanwhile,the cells in different growth stages also exhibited different performance advantages. Cells at the stationary growth phase exhibited better detection sensitivity and linearity,while cells in the logarithmic growth phase had lower limit of detection (LOD) . Moreover,there was an optimal induction time for the response of the sensor,overly long or short induction time could interfere with its response. The optimal induction time for the arsenic sensor in this work was about 2-3 h. By comparing three types of fluorescent protein reporters,it was found that although their detection limits were fairly similar,all within the range of 5-10μg/L,but their response times varied,ranging from 40 min to 2 h. The fluorescent proteins with higher brightness exhibited faster sensor response. These research outcomes provided a solid foundation for the practical application of microbes in detection. In practice,we could choose cells in specific states based on particular purpose,maximizing the performance of the cell sensors and further broadening the application scope of such sensors.
