Study on Spatial Distribution of Chemical Components in Flue Cured Tobacco Leaves by Imprinting Analytical Electrospray Photoionization Mass Spectrometry
10.19756/j.issn.0253-3820.231396
- VernacularTitle:印迹-解析电喷雾/光电离质谱成像技术研究烤烟叶中化学成分的空间分布
- Author:
Chun-Chun LYU
1
;
Yu-Ting JIANG
;
Yong-Hua HU
;
Liu-Tian WU
;
Ke-Ke QI
;
Cheng-Yuan LIU
;
Yang PAN
Author Information
1. 中国科学技术大学,国家同步辐射实验室,合肥230029
- Keywords:
Tobacco;
Mass spectrometry imaging;
Photoionization;
Imprint;
Chemical composition
- From:
Chinese Journal of Analytical Chemistry
2024;52(6):876-884,中插36-中插37
- CountryChina
- Language:Chinese
-
Abstract:
The imprint desorption electrospray photoionization mass spectrometry was employed to locally image the spatial distribution of chemical components in dried tobacco leaves after initial curing. The relative content distribution of different chemical components was obtained in tobacco leaves. The application of imprinting method could transfer tobacco internal compounds to the surface of porous polytetrafluoroethylene plate,which realized the detection and visual analysis of tobacco internal substances. Besides,the imprint desorption electrospray ionization/post-photoionization (Imprint DESI/PI) mass spectrometry imaging technique had the advantages of non-polarity discrimination,soft ionization and high ionization efficiency for plant samples,and could simultaneously detect and image rich compounds in tobacco samples. A total of 40 kinds of chemical components including alkaloids,amino acids,sugars,acids,ketones and phenols were identified based on high resolution mass spectrometry. The results showed that the representative chemical components of tobacco,such as alkaloids,amino acids and sugars,were mainly distributed near the leaf tip from the vertical analysis and at the left and right leaf edges from the horizontal analysis. Amadori compound (1-Deoxy-1-L-proline-d-fructose) was detected,and the content of Amadori was found to be consistent with that of free amino acid (proline). In addition,the technique was further used to study the climate spot disease area of tobacco,and it was found that the compounds had specific distribution in the climate spot area,which further proved the superiority of this method in studying the growth stress of tobacco leaves.
