Quantitative Analysis of Lithium Element in Whole Blood Using Laser-induced Breakdown Spectroscopy
10.19756/j.issn.0253-3820.231429
- VernacularTitle:激光诱导击穿光谱定量分析全血中的锂元素
- Author:
Wen-Xin REN
1
;
Liang YANG
;
Han ZHAO
;
Yi-Meng WANG
;
Da HUANG
;
Xin-Hua DAI
;
Qing-Yu LIN
;
Yi-Xiang DUAN
Author Information
1. 四川大学机械工程学院,成都 610065
- Keywords:
Laser-induced breakdown spectroscopy;
Blood;
Lithium element;
Quantitative analysis;
Partial least squares
- From:
Chinese Journal of Analytical Chemistry
2024;52(4):559-565
- CountryChina
- Language:Chinese
-
Abstract:
Lithium(Li)salts are commonly used as psychotropic medications for the treatment of major depressive disorders.However,long-term use of Li salts poses a high risk of toxicity,necessitating continuous monitoring of Li concentration in patient blood to ensure medication safety,which is crucial for clinical treatment.Laser-induced breakdown spectroscopy(LIBS),as a rapid analytical technique,has been widely applied in the elemental analysis of complex matrices in various practical scenarios.In this study,LIBS technology combined with partial least squares(PLS)was employed for quantitative analysis of Li elements in blood matrix.A total of 45 clinical blood samples were utilized,and the quantitative models for plasma and whole blood matrices were separately investigated.The number of latent variables in the PLS algorithm was optimized using a five-fold cross-validation method.Results revealed that the PLS quantitative model constructed on the basis of plasma matrix achieved a predictive determination coefficient(R2)of 0.992,a predictive root mean square error(RMSEP)of 0.204 μg/mL,and a relative standard error(RSD)of 2.14%.In contrast,for the PLS quantitative model constructed on the basis of whole blood matrix,the R2 was 0.984,the RMSEP was 0.728 μg/mL,and the RSD was 3.45%Consequently,the LIBS model constructed on the basis of plasma calibration values demonstrated superior performance in quantitative analysis of Li element in whole blood,and LIBS technology provided a new possibility for rapid assessment of blood Li levels in clinical practice,with promising prospects for application.
