Effect of salidroside on bone loss in rats with obstructive sleep apnea syndrome by regulating the OPG/RANKL pathway
10.16066/j.1672-7002.2024.08.010
- VernacularTitle:红景天苷调节OPG/RANKL通路对阻塞性睡眠呼吸暂停综合征大鼠骨质流失的影响
- Author:
Haibin MA
1
;
Zhaoxu YAO
;
Chuanjun WU
;
Lin LIU
;
Kaili SUN
;
Hui GONG
;
Wenyan ZHENG
Author Information
1. 邯郸市中心医院耳鼻咽喉头颈外一科,河北 邯郸 056000
- Keywords:
Sleep Apnea,Obstructive;
salidroside;
OPG/RANKL signaling pathway;
bone loss
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2024;31(8):526-530,533
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect of salidroside (Sal) on bone loss in obstructive sleep apnea syndrome(OSAS) rats by regulating the osteoprotegerin(OPG)/receptor activator of nuclear factor kappa-B ligand(RANKL) pathway. METHODS Rats were randomly divided into(12 rats/group) control group,OSAS group,Sal-L,Sal-M,and Sal-H groups(17.5,35,70 mg/kg). Except for the control group,all other groups were used to replicate the OSAS rat model through hypoxia and reoxygenation cycles. Bone density meters,three-point bending experiments,and Micro CT were applied to measure the bone density,biomechanics,and microstructural changes of the femur in rats. ELISA method was applied to detect serum levels of osteocalcin(BGP),alkaline phosphatase(ALP),and cross-linked carboxy-terminal telopeptide of type Ⅰ collagen(CTX-I). RT-PCR was applied to detect OPG and RANKL mRNA levels in the femur. Western blotting was applied to detect the expression of OPG/RANKL pathway proteins in the femur. RESULTS Compared with the control group,the bone density,maximum intensity,maximum load,trabecular bone volume fraction(Tb.BV/TV),trabecular number(Tb.N),trabecular thickness(Tb.Th),BGP,ALP,OPG mRNA and protein expression,OPG/RANKL ratio of rats in the OSAS group were decreased,the mRNA and protein expression of CTX-I and RANKL were increased(P<0.05). Compared with the OSAS group,the bone density,maximum intensity,maximum load,Tb.BV/TV,Tb.N,Tb.Th,BGP,ALP,OPG mRNA and protein expression,OPG/RANKL ratio of rats in the Sal-L,Sal-M,and Sal-H groups were increased sequentially,the mRNA and protein expression of CTX-I and RANKL were decreased sequentially,the above changes were most great in the Sal-H group(P<0.05). CONCLUSION Salidroside promotes bone formation and inhibits bone resorption by increasing OPG expression and decreasing RANKL expression,thereby reducing bone loss in OSAS rats.
