Correlation between inhibitory cytokines and disease severity in juvenile onset recurrent respiratory papillomatosis
10.16066/j.1672-7002.2024.06.010
- VernacularTitle:幼年型复发性呼吸道乳头状瘤病中抑制性细胞因子与疾病严重程度的相关性研究
- Author:
Shilan LI
1
;
Guixiang WANG
;
Guoliang WANG
;
Hua WANG
;
Wei WANG
Author Information
1. 国家儿童医学中心,首都医科大学附属北京儿童医院耳鼻咽喉头颈外科,北京 100045
- Keywords:
Laryngeal Neoplasms;
Interleukin-10;
human papillomavirus;
juvenile onset recurrent respiratory papillomatosis;
immunoregulatory cytokines
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2024;31(6):386-390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE Aims to investigate the expression changes of immunoregulatory cytokines in peripheral blood and local lesions of patients with juvenile onset recurrent respiratory papillomatosis(JORRP),and analyze their correlation with the severity of JORRP.METHODS Peripheral blood samples were collected from JORRP patients,along with peripheral blood samples from healthy children matched for gender and age as the control group.Papillomas of JORRP patients were collected,and the epithelial tissue from the palatine tonsils of patients undergoing tonsillectomy served as a control.ELISA was used to detect cytokine concentrations in plasma samples.The tissue samples were detected by mRNAseq and verified by RT-PCR.Furthermore,the correlation between cytokine expression levels and disease severity was analyzed.RESULTS The concentration of interleukin-10(IL-10)in the plasma of JORRP patients was significantly higher than that in the healthy control group(P<0.05),and it was negatively correlated with the age of onset(r=-0.3307,P<0.05).At the level of mRNA,the relative quantity of IL-10 in papillomas was significantly higher in the aggressive group compared to the non-aggressive group(P>0.05).Additionally,the concentration of TGF-β in plasma of JORRP patients was positively correlated with the number of surgeries(r=0.5144,P<0.05),and the concentration of TGF-β in the aggressive group was significantly higher than that in the non-aggressive group(P<0.05).The TGF-β mRNA expression level was significantly higher in the papillomas of JORRP patients compared to the control(P<0.001).CONCLUSION Inhibitory immunoregulatory cytokines IL-10 and TGF-β are overexpressed in JORRP patients and positively correlated with disease severity.
