The expression of TFF3 in chronic sinusitis with nasal polyps and its regulation on MUC5AC expression
10.16066/j.1672-7002.2024.06.009
- VernacularTitle:三叶因子家族肽3在慢性鼻窦炎伴鼻息肉中的表达及其对黏蛋白5AC表达的调控研究
- Author:
Liting SHAO
1
;
Huikang WANG
;
Zhaoyang LU
;
Zhaoxue ZHAI
;
Yu ZHANG
Author Information
1. 青岛大学附属烟台毓璜顶医院耳鼻咽喉头颈外科,山东省耳鼻喉疾病临床医学研究中心,烟台市耳鼻喉疾病重点实验室,山东烟台 264000
- Keywords:
Sinusitis;
Nasal Polyps;
trefoil factor family peptide;
mucin;
mucociliary clearance system
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2024;31(6):381-385
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the expression of trefoil factor family peptide(TFF3)in chronic rhinosinusitis with nasal polyps(CRSwNP)and its regulation on mucin 5AC(MUC5AC)expression.METHODS The nasal polyp tissues of 16 patients in the CRSwNP group and the nasal mucosal tissues of 16 patients in the control group were selected,and the expressions of TFF3 and MUC5AC were detected by quantitative real-time polymerase chain reaction(RT-qPCR)and Western blot,and the correlation between them was analyzed.The human nasal epithelial cell(HNEpC)line with TFF3 knockdown was constructed,and the expression of MUC5AC in KD-TFF3 HNEpC was detected by RT-qPCR and immunofluorescence.RESULTS The expression level of TFF3 in nasal polyps was significantly lower than that of control group,and the expression level of MUC5AC was increased,and the expression level of both was negatively correlated(r=-0.556,P<0.05).The expression of MUC5AC in KD-TFF3 HNEpC was significantly higher than that in control group.CONCLUSION The expression of TFF3 decreases in CRSwNP and negatively regulates the expression of MUC5AC.This study provides a new idea for the treatment of abnormal hypersecretion of mucous in chronic nasal inflammatory diseases represented by CRSwNP.
