Paraventricular hypothalamic nucleus projects directly to the preB?tzinger complex and is involved in regulation of respiration in mice
10.16016/j.2097-0927.202408088
- VernacularTitle:小鼠的下丘脑室旁核向前包钦格复合体直接投射参与调控呼吸
- Author:
Linglin LIU
1
;
Junhong ZHANG
;
Wei XIONG
Author Information
1. 400038 重庆,陆军军医大学(第三军医大学)第一附属医院老年医学与特勤医学科
- Keywords:
paraventricular hypothalamic nucleus;
preB?tzinger complex;
respiratory motor neural network;
phrenic motor neuron
- From:
Journal of Army Medical University
2024;46(20):2265-2275
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the spatial distribution of respiratory-related nuclei throughout the whole brain,and to investigate the circuit and projection links between the paraventricular hypothalamic nucleus (PVN)and the preB?tzinger complex (preB?tC)in mice.Methods Using a retrograde tracing strategy from peripheral to central,pseudorabies viruses (PRV)were microinjected into the diaphragm of mice to retrogradely label the upstream inputs brain regions from spinal respiratory phrenic motor neurons. The anterograde non-transsynaptic tracing viruses (AAV2/9-EGFP)were microinjected into the PVN to label the downstream brain region of the PVN;And using the Cre/loxP gene expression strategy,Dio-EGFP and Retro-Cre viruses were injected in the PVN and preB?tC,respectively,to validate the PVN-preB?tC circuit and the projection relationship between the two.Results The results of PRV-mediated retrograde tracing showed that PRV-EGFP-positive neurons were distributed in several brain regions such as PVN,preB?tC,nucleus tractus solitarii (NTS),locus coeruleus (LC)and periaqueductal grey (PAG).Except for the medulla oblongata region,the PVN brain region had the highest percentage of PRV-EGFP-positive neurons (P<0.0001 ).After AAV2/9-EGFP viruses were injected in the PVN,green neurofibrils were observed in the preB?tC brain region after viral infection;Dio-EGFP and Retro-Cre viruses were injected in the PVN and preB?tC,respectively,and green neuronal cells were seen in the brain region of the PVN after viral infection.Conclusion PVN is an important upstream input nucleus of spinal respiratory phrenic motor neurons,and has the potential to regulate respiration through direct projections from PVN-preB?tC circuit.
