Detection of Brucella abortus using an electrochemical immunosensor modified with PB-MWCNTs-GNPs
10.16016/j.2097-0927.202401080
- VernacularTitle:基于PB-MWCNTs-GNPs修饰的电化学免疫传感器检测流产布鲁氏菌
- Author:
Yu CHI
1
,
2
;
Yu CAO
;
Hao CHENG
;
Jingwen CAO
;
Jianyue AO
;
Haibo LI
;
Lei MA
;
Ming LIU
Author Information
1. 154007 黑龙江佳木斯,佳木斯大学临床医学院
2. 400038 重庆,陆军军医大学(第三军医大学)药学与检验医学系
- Keywords:
electrochemical immunosensor;
PB-MWCNTs-GNPs;
Brucella abortus
- From:
Journal of Army Medical University
2024;46(17):1969-1975
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a high performance electrochemical immunosensor for detecting Brucella abortus(B.abortus).Methods Prussian blue(PB),multi walled carbon nanotubes(MWCNTs)and gold nanoparticles(GNPs)(PB-MWCNTs-GNPs)nanocomposites were prepared,and appropriate antibody was used to construct the immunosensor for detecting B.abortus samples.The optimal conditions were clarified by examining the key factors in sensor construction,and then the performance of the sensor was evaluated.Results The optimal construction conditions were determined as follows:the ratio of MWCNTs-PB was 1∶5,the drying temperature was 37 ℃,the pH value of buffer system was 7.5,and the incubation time of antibody and sample was 1 h and 30 min,respectively.B.abortus exhibited a good linear relationship,when ranging from 10 to 1 × 105 CFU/mL.The sensor had good anti-interference ability,repeatability,stability and high accuracy.Conclusion Our prepared PB-MWCNTs GNPs nanomaterials modified electrochemical immunosensor for detecting B.abortus is easy to prepare,has good performance,and can provide reference for the early clinical diagnosis of brucellosis.
