Enamel matrix derivative improves remineralization of artificial root dentin caries in vitro
10.16016/j.2097-0927.202312108
- VernacularTitle:牙釉基质衍生物促进人工根面牙本质龋的再矿化
- Author:
Jin CHEN
1
,
2
;
Jing'ou LIANG
;
Linglin ZHANG
Author Information
1. 401120 重庆,重庆医科大学附属口腔医院附三院门诊部
2. 401147 重庆,重庆市口腔疾病与生物医学重点实验室,重庆市高校市级口腔生物医学工程重点实验室
- Keywords:
enamel matrix derivative;
root caries;
root dentin;
remineralization
- From:
Journal of Army Medical University
2024;46(16):1890-1896
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of enamel matrix derivative(EMD)on remineralization of artificial root dentin caries.Methods Bovine root dentin blocks were placed in a demineralization solution to create artificial root dentin carious lesions.The lesions were then randomly divided into EMD group(30 g/L EMD,the experimental group),sodium fluoride(NaF)aqueous solution group(1 g/L NaF,the positive control group)and distilled and deionised water(DDW)group(the negative control group),and all the lesions were pH-cycled at 6 cycles per day for 8 d.Morphology of the lesions were observed by scanning electron microscopy(SEM)and polarized light microscopy(PLM).Transverse microradiography(TMR)was used to examine mineral loss,lesion depth and mineral content.Results After pH-cycling,the surface morphology by SEM and the vertical morphology by PLM suggested that EMD and NaF treatment,but not DDW,caused mineral accumulation of artificial root dentin caries.Meanwhile,TMR revealed significantly lower mineral loss,shallower lesion depth and more mineral content in the EMD group than the DDW group(P＜0.05)after pH-cycling.Conclusion EMD can significantly promote remineralization of root dentin caries in vitro,and is a promising agent for non-invasive root caries therapy.
