Mechanism of glutaminergic neurons in medial prefrontal cortex involved in aggressive behavior of CD1 mice
10.16016/j.2097-0927.202312020
- VernacularTitle:内侧前额叶皮质谷氨酸能神经元参与CD1小鼠攻击行为的机制研究
- Author:
Jiaxin LI
1
;
Lu HUANG
;
Zonghong LONG
;
Min ZHANG
;
Huizhong WEN
;
Ying XIONG
;
Hong LI
;
Fuhai BAI
Author Information
1. 400038 重庆,陆军军医大学(第三军医大学):第二附属医院麻醉科
- Keywords:
medial prefrontal cortex;
glutamatergic neurons;
aggressive behavior
- From:
Journal of Army Medical University
2024;46(12):1336-1343
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the intrinsic neural mechanism of aggressive behavior in CD 1 mice.Methods CD1 mice with aggressive behavior were screened out by resident intruder test.After the aggressive conditioned pair preference was further verified,the activated brain regions of the whole brain were labeled with c-Fos,and the types of neurons activated by the aggressive behavior were analyzed by double immunofluorescence labeling.Finally,the effects of activity of these neurons regulated by optogenetics on aggressive behavior were observed.Results The c-Fos screening revealed that about 82％of the CD1 mice showed aggressive behavior.After the occurrence of aggressive behavior,the main activation occured in the medial prefrontal cortex(mPFC),and the results of immunofluorescence double labeling showed that the c-Fos positive neurons in the mPFC were mainly glutamatergic neurons.Finally,glutamatergic neurons in the mPFC could be activated by optogenetics,and the activation inhibited the aggressive behavior of CD1 mice.In contrast,optogenetics could inhibit glutamatergic neurons in the mPFC and then promote the aggressive behavior of CD1 mice.Conclusion Glutamatergic neurons in the mPFC are an important component in the regulation of aggressive behavior in CD1 mice.
