Infiltration and immunosuppressive function of tumor-associated B cells in gastric cancer patients
10.16016/j.2097-0927.202312006
- VernacularTitle:胃癌患者肿瘤组织中B细胞的浸润及其免疫抑制功能的研究
- Author:
Yuxian LI
1
;
Zhenquan DUAN
;
Ying WANG
;
Xueling TAN
;
Xiaohong YU
;
Yuanyuan ZHANG
;
Baohang ZHU
;
Yuan QIU
;
Liusheng PENG
;
Quanming ZOU
Author Information
1. 400038 重庆,陆军军医大学(第三军医大学)药学与检验医学系微生物与生化药学教研室
- Keywords:
gastric cancer;
B cells;
immunosuppression
- From:
Journal of Army Medical University
2024;46(9):1034-1040
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of B cells in both tumor and non-tumor tissues of gastric cancer patients,analyze their phenotypic characteristics and explore the impact on T cell proliferation.Methods Immunohistochemical staining was utilized to detect the expression of B cell surface marker CD 19 in tumor and non-tumor tissues from 33 gastric cancer patients.The expression levels of chemokine receptors and immunoglobulin molecules on B cells in both tumor and non-tumor tissues were measured using flow cytometry.Chemotaxis experiments were conducted to examine the role of the CXCL12-CXCR4 axis in B cell chemotaxis.B cells isolated and purified from both tissue types were co-cultured with autologous peripheral T cells to assess their effect on T cell proliferation.Results There were significantly more B cells infiltrated in tumor tissues than those infitrated in the non-tumor tissues of gastric cancer patients(P＜0.01),and CXCR4 was highly expressed on tumor-infiltrating B cells compared with B cells derived from non-tumor tissues(P＜0.05).The Cancer Genome Atlas(TCGA)analysis indicated that the expression level of CXCL12 in tumor tissues was positively correlated with the expression level of CD19 in gastric cancer patients(r=0.15,P＜0.01).And the expression level of CXCL12 in tumor tissues of the gastric cancer patients was also positively correlated with the number of B cells infiltrated in tumor tissues.Chemotaxis experiments confirmed that the CXCL12-CXCR4 axis was involved in promoting B cell chemotaxis(P＜0.05).Although B cells in tumor and non-tumor tissues had similar levels of IgM,IgG,and IgA expression,tumor-infiltrating B cells significantly inhibited the proliferation of T cells when compared with B cells derived from non-tumor tissues(P＜0.01).Conclusion There are more B cells infiltrated in gastric cancer tissues,which may be recruited to tumor tissues through the CXCL12-CXCR4 axis,and then inhibit T cell proliferation to promote the progression of gastric cancer.
