Characterization of CD8+T cell subsets in male and female non-obese diabetic mice
10.16016/j.2097-0927.202312058
- VernacularTitle:雌性和雄性非肥胖糖尿病小鼠中CD8+T细胞分化亚群差异研究
- Author:
Shushu YANG
1
;
Dong LIU
;
Jie LI
;
Mengjun ZHANG
;
Li WANG
Author Information
1. 400038 重庆,陆军军医大学(第三军医大学)基础医学院免疫学教研室
- Keywords:
type 1 diabetes;
CD8 positive T lymphocytes;
Treg cells;
exhausted T cells;
naive T cells;
memory T cells
- From:
Journal of Army Medical University
2024;46(8):837-848
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare and analyze the differences in CD8+naive,effector,memory,exhausted and regulatory T cells in order to investigate the impact of gender on the differentiation fate of CD8+T cells in the context of type 1 diabetes (T1D)based on female and male non-obese diabetic (NOD)mice and healthy Institute for Cancer Research (ICR)mice.Methods The frequencies and phenotypes of CD8+T cell differentiation subsets including naive T cells (TN),central memory T cells (TCM),effector T cells(TEFF),effector precursor T cells (TEP),exhausted T cells (TEX),precursor exhausted T cells (TPEX)and regulatory T cells (Tregs)in the spleen,pancreatic draining lymph nodes (pLN)and pancreas infiltrating lymphocytes (PIL)of male and female NOD mice were detected by flow cytometry.Results The frequencies of IFN-γ+,CD107a+and CCL5+CD8+TEFF in pLN and PIL of female NOD mice were significantly higher than those of male NOD mice.However,the frequencies of CD8+TN,CD8+TCM,CD8+TEX,CD8+TPEX and CD122+CD8+Tregs subsets in the spleen were significantly decreased.While there were no significant differences in the above CD8+T cell subsets except CD8+Tregs between female and male ICR mice. Conclusion Androgen may inhibit the differentiation of memory T cells into effector T cells and promote the exhaustion of effector T cells,leading to the difference in morbidity between the male and female mice.
