Liquid chromatography-mass spectrometry analysis for low-abundance protein polypeptides in vivo:research progress
10.16781/j.CN31-2187/R.20230643
- VernacularTitle:液相色谱-质谱法分析生物体内低丰度蛋白多肽的研究进展
- Author:
Shuwen GENG
1
,
2
;
Wen YANG
;
Junqin MAO
;
Tingting ZHOU
Author Information
1. 海军军医大学(第二军医大学)药学系药物分析教研室,上海 200433
2. 上海市第一人民医院嘉定医院(上海市嘉定区江桥医院)临床药学科,上海 201803
- Keywords:
protein polypeptides;
liquid chromatography-mass spectrometry;
sample pretreatment;
signal conversion and amplification
- From:
Academic Journal of Naval Medical University
2024;45(10):1296-1303
- CountryChina
- Language:Chinese
-
Abstract:
Protein polypeptides are a class of bioactive substances that play a crucial role in maintaining the stability of various functions of the organism.Rapid and accurate detection of their levels could help in the diagnosis of diseases,the monitoring of drug therapy and the research and development of medicines,which is of great significance in the fields of clinical medicine,biology and pharmacy.Conventional protein polypeptides detection methods,such as Western blotting and enzyme-linked immunosorbent assay,still have problems like low sensitivity or difficulty in determining more than 1 analyte simultaneously.Liquid chromatography-mass spectrometry(LC-MS)has the advantages of specificity,sensitivity and high throughput.However,low ionization efficiency of macromolecules and strong biological matrix effects limit the feasibility of direct detection of protein polypeptides by LC-MS,which has led to the development of signal conversion and amplification strategies based on LC-MS technology.In this review,we summarized the research progress of sample pretreatment methods and signal conversion and amplification strategies for quantification of protein polypeptides in vivo based on LC-MS in recent years,providing a reference for developing specific high-sensitivity detection methods for low-abundance protein polypeptides in complex samples based on LC-MS technology.
