Establishment and identification of a Trappc11 inducible knockout mouse model
10.16781/j.CN31-2187/R.20230302
- VernacularTitle:Trappc11诱导型基因敲除小鼠模型的构建及鉴定
- Author:
Bobo WANG
1
;
Meng GONG
;
Jing WEN
;
Xiaoli ALUS
;
Youlei LI
Author Information
1. 延安大学医学院生理学教研室,延安 716000
- Keywords:
trafficking protein particle complex subunit 11;
gene knockout mice;
CRISPR/Cas9 system;
Cre-loxP system;
tamoxifen
- From:
Academic Journal of Naval Medical University
2024;45(9):1156-1161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an inducible knockout mouse model of trafficking protein particle complex subunit 11 (Trappc11). Methods and results LoxP sites were introduced on both sides of exon 3-5 of Trappc11,and then the CRISPR/Cas9 technique was used to establish F0 C57BL/6J mice. The positive F0 generation mice were identified by polymerase chain reaction amplification and sequencing. After that,F0 positive mice were mated with C57BL/6J wild type mice to obtain F1 Trappc11flox/+mice. And then,Trappc11flox/+mice were mated with UBC-CreERT2 mice,and finally Trappc11 inducible systemic knockout mouse model was obtained after 2 generations. Conclusion The Trappc11 inducible knockout mouse model is established using CRISPR/Cas9 and Cre-loxP,providing an important tool for revealing the pathophysiological role of Trappc11 in multi-organ system diseases.
