Study on effect of LINC00342 in cervical cancer
10.3969/j.issn.1671-8348.2024.21.007
- VernacularTitle:LINC00342在宫颈癌中的作用研究
- Author:
Mi GONG
1
;
Yuchen SONG
;
Shanshan QIN
;
Ting WANG
;
Yijun YANG
Author Information
1. 徐州医科大学淮安临床学院妇科,江苏淮安 223300
- Keywords:
cervical cancer;
LINC00342;
proliferation;
invasion;
migration
- From:
Chongqing Medicine
2024;53(21):3233-3239
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of long non-coding RNA-LINC00342 in cervical cancer.Methods The bioinformatic analysis was performed by the cervical cancer data sets(GSE6791 and GSE63514)in GEO database.The potential oncogene LINC00342 was screened out.qRT-PCR was adopted to detect the expression level of LINC00342 in cervical cancer tissue and paracancerous normal cervical tissues of 50 cases of cervical cancer,and its relationship with the prognosis of the patients was analyzed.The correlation between LINC00342 with the other pathological features in the patients with cervical cancer was analyzed.In the cervical cancer cell lines Hela and SiHa cells,after transfecting si-LINC00342 and si-NCS,the LINC00342 expression level in the cells was detected by qRT-PCR.The cellular invasive and migration were detected by CCK-8/EdU proliferation test,Transwell invasion and migration test.Results The data sets of GSE6791 and GSE63514 and clinical tissue samples all showed that LINC00342 was highly expressed in cervical cancer tis-sues(P＜0.05).The clinical tissue samples related tests showed that the expression level of LINC00342 was correlated with the prognosis of the patients(HR=4.911,P=0.036 1);the LINC00342 level had no relation with the factors such as age of the patients and tumor histological type(P＞0.05),but was closely related to the tumor size and tumor stage(P＜0.05).The cellular experiments showed that knocking down LINC00342 expression could significantly inhibit the proliferation,invasion and migration abilities of cervical cancer cells(P＜0.05).Conclusion LINC00342 is highly expressed in cervical cancer tissues,which is a potential biomar-ker of the occurrence,development and poor prognosis of cervical cancer.
