Rapid enzyme-free colorimetric analysis of alpha-fetoprotein by combining triple-helix molecular probe and catalytic hairpin assembly reaction
10.3969/j.issn.1671-8348.2024.19.003
- VernacularTitle:结合三螺旋分子探针和催化发夹组装反应对甲胎蛋白的快速无酶比色分析
- Author:
Qin WENG
1
;
Yan DONG
;
Gang BAI
;
Xiaodong LEI
;
Hua YU
Author Information
1. 成都中医药大学医学与生命科学学院,成都 611137
- Keywords:
hepatocellular carcinoma;
alpha-fetoprotein;
triple-helix molecular probe;
catalytic hairpin assembly;
colorimetric analysis
- From:
Chongqing Medicine
2024;53(19):2892-2897,2906
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a novel enzyme-free colorimetric biosensor(T-CHA)based on tri-ple-helix molecular probe(THMP)and catalytic hairpin assembly(CHA)reaction for visual detection and precise quantitative analysis of low-abundance alpha-fetoprotein(AFP).Methods The absorbance produced by AFP induced T-CHA system was recorded by gel electrophoresis and ultraviolet-visible spectrophotometer to evaluate the feasibility of T-CHA strategy.The molar concentration ratio of THMP and CHA,reaction time of CHA,temperature and pH value of buffer solution were optimized respectively.The color reaction of T-CHA was induced by different concentrations of AFP to explore the detection efficiency of T-CHA.Results Under the op-timal experimental conditions,the logarithmic values of AFP at different concentrations showed a linear rela-tionship from 5 μg/mL to 10 ng/mL,and the detection limit was 2.29 μg/mL.The T-CHA system could com-plete accurate quantitative analysis of low abundance AFP and visual free-labeled detection within 105 min,moreover the sensitivity of T-CHA was superior to that of ELISA.Conclusion Utilizing the low background leakage of THMP and the high catalytic efficiency of CHA,the T-CHA system could realize the early accurate diagnosis of hepatocellular carcinoma,moreover which has a certain application prospect in the real time detec-tion.
