Study on inhibitory effect of Qiangxin Capsule on fibrosis caused by Wnt/β-catenin signaling pathway in SD heart failure rats
10.3969/j.issn.1671-8348.2024.19.002
- VernacularTitle:强心胶囊对SD心力衰竭大鼠Wnt/β-catenin信号通路导致纤维化的抑制效果研究
- Author:
Yang QU
1
;
Yibin DU
;
Biqin CHEN
;
Qianyun LI
Author Information
1. 云南中医药大学第一附属医院,昆明 650021
- Keywords:
Qiangxin Capsule;
heart failure;
Wenyang Lishui;
cardiac fibrosis;
Wnt/β-catenin;
ventricu-lar remodeling
- From:
Chongqing Medicine
2024;53(19):2887-2891
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect and related mechanisms of Qiangxin Capsule on fi-brosis caused by Wnt(wingless)/β-catenin signaling pathway in Sprague Dawley(SD)heart failure(HF)rats.Methods Fifteen SPF-grade SD male rats aged 6-8 weeks were selected as the research subjects.The rats were randomly divided into the sham operation group,model group and medication group,5 cases in each group.The model group and medication group caused abdominal aorta partial stenosis by operation.The rats in the sham operation group and model group were gavaged by 10 mL/kg of normal saline,the rats in the medication group were gavaged by 0.8 g/kg Qiangxin Capsule,and each group was continuously administered for 20 weeks.The ventricular wall motion amplitude was used to detect the left ventricular ventricular ejection fraction(LVEF),left ventricular end-systolic diameter(LVEDs)and left ventricular end-diastolic diameter(LVEDd)in each group.After 20 weeks,the rats in each group were executed,the rat myocardial tissues were taken for conducting the pathological analysis,the myocardial tissues were observed by hematoxylin-eosin(HE)staining,the fibrous tissues were observed by the Masson staining,and the protein expression levels of Wnt,β-catenin,collagen Ⅰ and collagen Ⅲ were detected by Western blot.Results LVEF in the medication group was higher than that in the model group,LVEDd and LVEDs were smaller than those in the model group,and the differences were statistically significant(P＜0.05).Compared with the model group,the Wnt/GAPDH and β-catenin/GAPDH levels in the medication group were decreased,and the difference was statisti-cally significant(P＜0.05).Compared with the model group,the collagen Ⅰ/GAPDH and collagen Ⅲ/GAP-DH in the medication group were decreased,and the difference was statistically significant(P＜0.05).Conclu-sion Qiangxin Capsule could alleviate the cardiac fibrosis degree in SD rats with heart failure after abdominal aortal coarctation,and its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.
