Regulation of NFATC2 on growth of ovarian cancer cells and possible mechanism
10.3969/j.issn.1671-8348.2024.13.004
- VernacularTitle:NFATc2对卵巢癌细胞的生长调控及其机制研究
- Author:
Mingju HUANG
1
;
Jianhua ZENG
Author Information
1. 重庆大学附属三峡医院妇科,重庆 404000
- Keywords:
ovarian neoplasms;
nuclear factor of activated T cells;
CXC chemokine motif ligand 8;
pro-liferation;
apoptosis
- From:
Chongqing Medicine
2024;53(13):1941-1946,1951
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of nuclear factor of activated T cells 2(NFATc2)on the proliferation and apoptosis of human ovarian cancer cells and its possible mechanism.Methods The human epithelial ovarian cancer cell line A2780 was selected,and the A2780 cell lines with over expression(over ex-pression group)and low expression[transfection of small interfering RNA(siRNA),NFATc2 siRNA group]of NFATc2 were constructed,respectively.The control group without any treatment was established.Thiazole blue(MTT)and flow cytometry were used to detect the cell proliferation and apoptosis levels,respectively.The expression levels of apoptosis related proteins and CXC chemokine motif ligand 8(CXCL8)were detected by Western blot.Normal A2780 cells(the blank group),A2780 cells transfected with blank vectors(the con-trol group)and A2780 cells with low expression of NFATc2(the NFATc2 siRNA group)were used to estab-lish ovarian cancer model with subcutaneous xenograft.The tumor volume and mass were measured,and the expression level of CXCL8 in tumor tissue was detected by Western blot.Results Compared with the control group,the cell proliferation rate in the NFATc2 siRNA group was decreased,the cellular apoptosis rate was increased,the cleaved caspase-3 expression level was increased,the Bcl-2/Bax ratio was decreased,the CXCL8 expression was decreased,and the differences were statistically significant(P＜0.01).The cell lines in the o-ver expression group showed the opposite effect.The tumor mass and volume of nude mice,and the expression level of CXCL8 in the NFATc2 siRNA group were lower than those in the blank group and the control group(P＜0.01).Conclusion Down-regulation of NFATc2 could reduce the cell proliferation,increase the expres-sion of apoptosis related proteins by inhibiting CXCL8 expression in A2780 cells,thus promote the apoptosis of ovarian cancer cells.
